Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, SH-SY5Y cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13585-1-AP targets PRDX6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PRDX6 fusion protein Ag4512|
|Full Name||peroxiredoxin 6|
|Calculated molecular weight||224 aa, 25 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC035857|
|Gene ID (NCBI)||9588|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).
|Product Specific Protocols|
|WB protocol for PRDX6 antibody 13585-1-AP||Download protocol|
|IHC protocol for PRDX6 antibody 13585-1-AP||Download protocol|
|IF protocol for PRDX6 antibody 13585-1-AP||Download protocol|
|IP protocol for PRDX6 antibody 13585-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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