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PRDX6 Polyclonal antibody
PRDX6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13585-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SH-SY5Y cells, A549 cells, HeLa cells, HAP1, K-562 cells, HepG2 cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | HeLa cells, HAP1 cells |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HAP1 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 5 publications below |
WB | See 21 publications below |
IHC | See 5 publications below |
IF | See 2 publications below |
Product Information
13585-1-AP targets PRDX6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PRDX6 fusion protein Ag4512 |
Full Name | peroxiredoxin 6 |
Calculated molecular weight | 224 aa, 25 kDa |
Observed molecular weight | 25-30 kDa |
GenBank accession number | BC035857 |
Gene symbol | PRDX6 |
Gene ID (NCBI) | 9588 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).
Protocols
Product Specific Protocols | |
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WB protocol for PRDX6 antibody 13585-1-AP | Download protocol |
IHC protocol for PRDX6 antibody 13585-1-AP | Download protocol |
IF protocol for PRDX6 antibody 13585-1-AP | Download protocol |
IP protocol for PRDX6 antibody 13585-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance. | ||
Nat Commun Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs.
| ||
Br J Pharmacol Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation. | ||
Antioxid Redox Signal Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway. | ||
Environ Toxicol Pharmacol 4-tert-octylphenol injures motility and viability of human sperm by affecting cAMP-PKA/PKC-tyrosine phosphorylation signals. | ||
Front Oncol Identification of a Novel Ferroptosis-Related Gene Prognostic Signature in Bladder Cancer. |