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  • KD/KO Validated

PRDX6 Polyclonal antibody

PRDX6 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13585-1-AP

Synonyms

1 Cys, 1 Cys peroxiredoxin, 1 Cys PRX, 24 kDa protein, aiPLA2, Antioxidant protein 2, AOP2, KIAA0106, Liver 2D page spot 40, NSGPx, p29, peroxiredoxin 6, PRDX6, PRX, Red blood cells page spot 12



Tested Applications

Positive WB detected inSH-SY5Y cells, A549 cells, HeLa cells, HAP1, K-562 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells, HAP1 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in48孔 HepG2 cells, HAP1

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13585-1-AP targets PRDX6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX6 fusion protein Ag4512
Full Name peroxiredoxin 6
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC035857
Gene Symbol PRDX6
Gene ID (NCBI) 9588
RRIDAB_2168637
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

Protocols

Product Specific Protocols
WB protocol for PRDX6 antibody 13585-1-APDownload protocol
IHC protocol for PRDX6 antibody 13585-1-APDownload protocol
IF protocol for PRDX6 antibody 13585-1-APDownload protocol
IP protocol for PRDX6 antibody 13585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.

Authors - Haohao Huang
humanWB

Nat Commun

Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs.

Authors - Nisebita Sahu
  • KO Validated
ratWB

Br J Pharmacol

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Authors - Bo Wei
humanWB

Antioxid Redox Signal

Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway.

Authors - Shuaipeng Ma
ratWB,IHC

Environ Sci Pollut Res Int

Curcumin strengthens a spontaneous self-protective mechanism-SP1/PRDX6 pathway, against di-n-butyl phthalate-induced testicular ferroptosis damage

Authors - Hengtao Bu
humanWB

Environ Toxicol Pharmacol

4-tert-octylphenol injures motility and viability of human sperm by affecting cAMP-PKA/PKC-tyrosine phosphorylation signals.

Authors - Shaoping Huang