PRDX6 Polyclonal antibody

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  WB analysis using 13585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13585-1-AP

  WB result of PRDX6 antibody (13585-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells.

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  WB analysis of HAP1 using 13585-1-AP

  HAP1 (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 13585-1-AP incubated at 1:2000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis using 13585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13585-1-AP

  IP result of anti-PRDX6 (IP:13585-1-AP, 3ug; Detection:13585-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IP experiment of HAP1 using 13585-1-AP

  HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 13585-1-AP coupled to protein A- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human brain using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13585-1-AP (PRDX6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13585-1-AP (PRDX6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13585-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using PRDX6 antibody (13585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13585-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRDX6 antibody (13585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HAP1 using 13585-1-AP

  HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 13585-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• Featured Product
• KD/KO Validated

PRDX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13585-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:2.3.1.23, EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 1-Cys PRX

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, A549 cells, HeLa cells, HAP1, K-562 cells, HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells, HAP1 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: 48孔 HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 32 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Product Information

13585-1-AP targets PRDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRDX6 fusion protein Ag4512
• Full Name: peroxiredoxin 6
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC035857
• Gene Symbol: PRDX6
• Gene ID (NCBI): 9588
• RRID: AB_2168637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30041
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice; Authors - Wen Yin
human	WB	Nat Commun; Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.; Authors - Haohao Huang
human	WB	Nat Commun; Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs.; Authors - Nisebita Sahu ; KO Validated
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
human	WB	Biomed Pharmacother; Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis; Authors - Qiuyan Guo
human	WB	Antioxid Redox Signal; Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway.; Authors - Shuaipeng Ma