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  • KD/KO Validated

PRDX6 Polyclonal antibody

PRDX6 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13585-1-AP

Synonyms

1 Cys, 1 Cys peroxiredoxin, 1 Cys PRX, 24 kDa protein, aiPLA2, Antioxidant protein 2, AOP2, KIAA0106, Liver 2D page spot 40, NSGPx, p29, peroxiredoxin 6, PRDX6, PRX, Red blood cells page spot 12



Tested Applications

Positive WB detected inA549 cells, HeLa cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13585-1-AP targets PRDX6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX6 fusion protein Ag4512
Full Name peroxiredoxin 6
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC035857
Gene symbol PRDX6
Gene ID (NCBI) 9588
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

Protocols

Product Specific Protocols
WB protocol for PRDX6 antibody 13585-1-APDownload protocol
IHC protocol for PRDX6 antibody 13585-1-APDownload protocol
IF protocol for PRDX6 antibody 13585-1-APDownload protocol
IP protocol for PRDX6 antibody 13585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Rep

The role of cytochrome P450 2E1 on ethanol-mediated oxidative stress and HIV replication in human monocyte-derived macrophages.

Authors - Yuqing Gong
humanWB

Acta Pharmacol Sin

Identification of PRDX6 as a regulator of ferroptosis.

Authors - Bin Lu
  • KD Validated
ratWB

Br J Pharmacol

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Authors - Bo Wei
humanWB

Environ Toxicol Pharmacol

4-tert-octylphenol injures motility and viability of human sperm by affecting cAMP-PKA/PKC-tyrosine phosphorylation signals.

Authors - Shaoping Huang
ratWB

Artif Organs

Hypothermic oxygenated perfusion with defatting cocktail further improves steatotic liver grafts in a transplantation rat model.

Authors - Fan Lin
ratWB

Endocrine

Chronic angiotensin receptor activation promotes hepatic triacylglycerol accumulation during an acute glucose challenge in obese-insulin-resistant OLETF rats.

Authors - Jose A Godoy-Lugo