CoraLite® Plus 488-conjugated PRDX6 Polyclonal antibody

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  IF Staining of HeLa using CL488-13585

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PRDX6 antibody (CL488-13585) at dilution of 1:500.

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  FC experiment of HeLa using CL488-13585

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PRDX6 (CL488-13585) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

PRDX6 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-13585

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

EC:2.3.1.23, EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 1-Cys PRX

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13585 targets PRDX6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4512

  Product name: Recombinant human PRDX6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-224 aa of BC035857

  Sequence: MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP

• Full Name: peroxiredoxin 6
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC035857
• Gene Symbol: PRDX6
• Gene ID (NCBI): 9588
• RRID: AB_3083944
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30041
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).