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PKC Alpha Polyclonal antibody

PKC Alpha Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 21991-1-AP


AAG6, PKC A, PKC alpha, PKCA, PKCα, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, Jurkat cells, rat kidney tissue, human peripheral blood platelets
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman thyroid cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, NIH/3T3 cells, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21991-1-AP targets PKC Alpha in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Alpha fusion protein Ag17275
Full Name protein kinase C, alpha
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberAK055431
Gene Symbol PRKCA
Gene ID (NCBI) 5578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.


Product Specific Protocols
WB protocol for PKC Alpha antibody 21991-1-APDownload protocol
IHC protocol for PKC Alpha antibody 21991-1-APDownload protocol
IF protocol for PKC Alpha antibody 21991-1-APDownload protocol
IP protocol for PKC Alpha antibody 21991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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ATF4/CEMIP/PKCα promotes anoikis resistance by enhancing protective autophagy in prostate cancer cells.

Authors - Ying Yu

Cell Death Differ

β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.

Authors - Jia Ma


β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.

Authors - Yanwei Lu


Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth

Authors - Chunming Gu

Front Pharmacol

Cellular mechanism of action of forsythiaside for the treatment of diabetic kidney disease

Authors - Chunmei Xu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Alessandro (Verified Customer) (11-07-2022)

no unspecific staining, great outcome

  • Applications: Immunofluorescence

Chiara (Verified Customer) (10-04-2021)

Works very well in Immunoblot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: mesenchymal Stem cell

Samantha (Verified Customer) (02-24-2020)

Bovine vascular smooth muscle cells were transfected with either control siRNA or PKCalpha siRNA for up to 7 days, with transfections repeated every 48-72 hours. Blocking agent: 5% (w/v) milk in TBST for 1hrPrimary antibody diluted in 5% (w/v) milk in TBST; incubated overnight at 4 degrees.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Bovine vascular smooth muscle cells
PKC Alpha Antibody Western Blot, validation (1:750 dilution) in Bovine vascular smooth muscle cells (Cat no:21991-1-AP)

Ryan (Verified Customer) (01-10-2018)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina
PKC Alpha Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina (Cat no:21991-1-AP)