pan-PKC Polyclonal antibody

pan-PKC Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG


human, mouse, rat, zebrafish





Cat no : 12919-1-AP


pan-PKC, PKC B, PKC beta, PKCB, PRKCB, PRKCB1, PRKCB2, Protein kinase C beta type, protein kinase C, beta

Tested Applications

Positive WB detected inK-562 cells, mouse thymus tissue, human peripheral blood platelets, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inzebrafish retina
Positive FC detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:150-1:600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12919-1-AP targets pan-PKC in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen pan-PKC fusion protein Ag3591
Full Name protein kinase C, beta
Calculated Molecular Weight 673 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC036472
Gene Symbol PRKCB
Gene ID (NCBI) 5579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.


Product Specific Protocols
WB protocol for pan-PKC antibody 12919-1-APDownload protocol
IHC protocol for pan-PKC antibody 12919-1-APDownload protocol
IP protocol for pan-PKC antibody 12919-1-APDownload protocol
FC protocol for pan-PKC antibody 12919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Hematol Oncol

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Authors - Ning Xu

Am J Respir Cell Mol Biol

Intermittent Hypoxia Augments Pulmonary Vasoconstrictor Reactivity through PKCβ/Mitochondrial Oxidant Signaling.

Authors - Jessica B Snow
  • KD Validated

J Cell Mol Med

Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway.

Authors - Luo Wenjuan W

Oxid Med Cell Longev

p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.

Authors - Ming Zhang

J Cell Mol Med

Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization.

Authors - Jing Cui

Reprod Biol Endocrinol

In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein

Authors - Jiaqi Tang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Ashish (Verified Customer) (05-21-2021)

This antibody gave very clear staining results in lung tissue. Very nice antibody to work.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Lung
pan-PKC Antibody Immunohistochemistry validation (1:300 dilution) in Lung (Cat no:12919-1-AP)

Amy (Verified Customer) (09-06-2019)

This antibody dilution gave very clear protein bands in all murine colon samples used to date. Following ECL addition, the imager took ~240 seconds to detect PKCbeta (automatic exposure). Very easy antibody to work with

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Murine Colon Samples