PKC Beta Polyclonal antibody

PKC Beta Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 12919-1-AP

Synonyms

PKC B, PKC beta, PKCB, PRKCB, PRKCB1, PRKCB2, Protein kinase C beta type, protein kinase C, beta



Tested Applications

Positive WB detected inK-562 cells, human peripheral blood platelets, mouse thymus tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12919-1-AP targets PKC Beta in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Beta fusion protein Ag3591
Full Name protein kinase C, beta
Calculated molecular weight 673 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC036472
Gene symbol PRKCB
Gene ID (NCBI) 5579
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

Protocols

Product Specific Protocols
WB protocol for PKC Beta antibody 12919-1-APDownload protocol
IHC protocol for PKC Beta antibody 12919-1-APDownload protocol
IP protocol for PKC Beta antibody 12919-1-APDownload protocol
FC protocol for PKC Beta antibody 12919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Mol Histol

Effect of corticision on orthodontic tooth movement in a rat model as assessed by RNA sequencing.

Authors - Qihui Gu

Evid Based Complement Alternat Med

Revealing Synergistic Mechanism of Multiple Components in Gandi Capsule for Diabetic Nephropathy Therapeutics by Network Pharmacology.

Authors - Jian Zhang

Oncol Rep

PMID: 27748887

Authors - Jian-Feng Wu
humanWB

Sci Rep

Eupolyphaga sinensis Walker displays inhibition on hepatocellular carcinoma through regulating cell growth and metastasis signaling.

Authors - Yanmin Zhang
ratWB

Life Sci

Prenatal hypoxia attenuated contraction of offspring coronary artery associated with decreased PKCβ Ser660 phosphorylation and intracellular calcium.

Authors - Xueyi Chen
ratWB

J Endocrinol

In utero hypoxia altered Ang II-induced contraction via PKCβ in fetal cerebral arteries.

Authors - Hongyu Su

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ashish (Verified Customer) (05-21-2021)

This antibody gave very clear staining results in lung tissue. Very nice antibody to work.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Lung
PKC Beta Antibody Immunohistochemistry validation (1:300 dilution) in Lung (Cat no:12919-1-AP)
FH

Amy (Verified Customer) (09-06-2019)

This antibody dilution gave very clear protein bands in all murine colon samples used to date. Following ECL addition, the imager took ~240 seconds to detect PKCbeta (automatic exposure). Very easy antibody to work with

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Murine Colon Samples