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CoraLite® Plus 488-conjugated PKC Beta Polyclonal antibody

PKC Beta Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12919

Synonyms

PKC B, PKC beta, PKCB, PRKCB, PRKCB1, PRKCB2, Protein kinase C beta type, protein kinase C, beta

Validation Data Gallery

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  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated PKC Beta Polyclonal (CL488-12919)

    FC experiment of K-562 using CL488-12919

    1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PKC Beta (CL488-12919) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12919 targets PKC Beta in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Beta fusion protein Ag3591
Full Name protein kinase C, beta
Calculated Molecular Weight 673 aa, 77 kDa
GenBank Accession NumberBC036472
Gene Symbol PRKCB
Gene ID (NCBI) 5579
RRIDAB_2919063
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 PKC Beta antibody CL488-12919Download protocol
Standard Protocols
Click here to view our Standard Protocols