CoraLite®488-conjugated PKC Beta Polyclonal antibody

PKC Beta Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-12919

Synonyms

PKC B, PKC beta, PKCB, PRKCB, PRKCB1, PRKCB2, Protein kinase C beta type, protein kinase C, beta



Tested Applications

Positive FC detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 12919-1-AP

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-12919 targets PKC Beta in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Beta fusion protein Ag3591
Full Name protein kinase C, beta
Calculated molecular weight 673 aa, 77 kDa
GenBank accession numberBC036472
Gene symbol PRKCB
Gene ID (NCBI) 5579
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

Protocols

Product Specific Protocols
FC protocol for CL488 PKC Beta antibody CL488-12919Download protocol
Standard Protocols
Click here to view our Standard Protocols