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PRMT1 Polyclonal antibody

PRMT1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11279-1-AP

Synonyms

ANM1, HCP1, HMT2, HRMT1L2, IR1B4, PRMT1



Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
Positive IP detected inA549 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11279-1-AP targets PRMT1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT1 fusion protein Ag1790
Full Name protein arginine methyltransferase 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC019268
Gene Symbol PRMT1
Gene ID (NCBI) 3276
RRIDAB_2171319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

Protocols

Product Specific Protocols
WB protocol for PRMT1 antibody 11279-1-APDownload protocol
IHC protocol for PRMT1 antibody 11279-1-APDownload protocol
IF protocol for PRMT1 antibody 11279-1-APDownload protocol
IP protocol for PRMT1 antibody 11279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IP

Cell Metab

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners

Authors - Qing Li
humanWB

Cell Death Dis

PRMT1-mediated PGK1 arginine methylation promotes colorectal cancer glycolysis and tumorigenesis

Authors - Hao Liu
humanIHC

Oncogene

PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.

Authors - Xin-Ke Yin
human,mouseWB,IHC

NPJ Precis Oncol

The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer

Authors - Xiangwei Zeng
mouseWB

Diabetes

PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ

Authors - Yi Zhong
mouseWB,IF

Front Mol Neurosci

Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein

Authors - Cheng Wu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HUVECs