Validation Data Gallery
|Positive WB detected in||HeLa cells, Raji cells, mouse embryo tissue, Jurkat cells, HepG2 cells, NIH/3T3 cells|
|Positive IHC detected in||human breast cancer tissue, human lung tissue, human colon cancer tissue, human lymphoma tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18436-1-AP targets PRMT5 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PRMT5 fusion protein Ag9736|
|Full Name||protein arginine methyltransferase 5|
|Calculated molecular weight||637 aa, 73 kDa|
|Observed molecular weight||72 kDa|
|GenBank accession number||BC025979|
|Gene ID (NCBI)||10419|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.
Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response.
The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.
USP9X regulates centrosome duplication and promotes breast carcinogenesis.
Aging (Albany NY)
High PRMT5 expression is associated with poor overall survival and tumor progression in bladder cancer.
Exp Ther Med
Protein arginine methyltransferase 5 mediates THP-1-derived macrophage activation dependent on NF-κB in endometriosis.
Cell Death Dis
MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Satyendra (Verified Customer) (07-16-2021)
I got really nice band of PRMT5 Knock out and knockdown