PRMT5 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRMT5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 18436-1-AP

Synonyms

Protein arginine N-methyltransferase 5, Protein arginine N methyltransferase 5, Jak-binding protein 1, Histone-arginine N-methyltransferase PRMT5, EC:2.1.1.320

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Validation Data Gallery

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  WB analysis using 18436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 18436-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18436-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 18436-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 18436-1-AP

  IP result of anti-PRMT5 (IP:18436-1-AP, 4ug; Detection:18436-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of mouse liver using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lymphoma using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18436-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRMT5 antibody (18436-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 18436-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRMT5 antibody (18436-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 18436-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PRMT5 (18436-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells, mouse embryo tissue, Raji cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human lymphoma tissue, human lung tissue, human colon cancer tissue, human lung cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IP: See 6 publications below
• CoIP: See 1 publications below

Product Information

18436-1-AP targets PRMT5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRMT5 fusion protein Ag9736
• Full Name: protein arginine methyltransferase 5
• Calculated Molecular Weight: 637 aa, 73 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC025979
• Gene Symbol: PRMT5
• Gene ID (NCBI): 10419
• RRID: AB_2171798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.

Publications

Species	Application	Title
human	WB	Sci Adv; Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response.; Authors - Dapeng Ma ; KD Validated
human	WB	Nat Commun; The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.; Authors - Zhe Li
human	WB,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
human	WB,IP	Cell Death Dis; MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma; Authors - Jinrong Zhu
human	WB,IHC	Aging (Albany NY); High PRMT5 expression is associated with poor overall survival and tumor progression in bladder cancer.; Authors - Lei Tan ; KD Validated
human	WB	Exp Ther Med; Protein arginine methyltransferase 5 mediates THP-1-derived macrophage activation dependent on NF-κB in endometriosis.; Authors - Xiaoshan Chai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Satyendra (Verified Customer) (07-16-2021)

I got really nice band of PRMT5 Knock out and knockdown

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293

PRMT5 Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:18436-1-AP)

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