• Featured Product
  • KD/KO Validated

PRMT5 Polyclonal antibody

PRMT5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18436-1-AP

Synonyms

72 kDa ICln binding protein, HRMT1L5, IBP72, Jak binding protein 1, JBP1, PRMT5, SKB1, SKB1 homolog, SKB1Hs



Tested Applications

Positive WB detected inHeLa cells, Raji cells, mouse embryo tissue, Jurkat cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human lung tissue, human colon cancer tissue, human lymphoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18436-1-AP targets PRMT5 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT5 fusion protein Ag9736
Full Name protein arginine methyltransferase 5
Calculated molecular weight 637 aa, 73 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC025979
Gene symbol PRMT5
Gene ID (NCBI) 10419
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.

Protocols

Product Specific Protocols
WB protocol for PRMT5 antibody 18436-1-APDownload protocol
IHC protocol for PRMT5 antibody 18436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response.

Authors - Dapeng Ma
  • KD Validated
humanWB

Nat Commun

The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.

Authors - Zhe Li
humanWB,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
humanWB,IHC

Aging (Albany NY)

High PRMT5 expression is associated with poor overall survival and tumor progression in bladder cancer.

Authors - Lei Tan
  • KD Validated
humanWB

Exp Ther Med

Protein arginine methyltransferase 5 mediates THP-1-derived macrophage activation dependent on NF-κB in endometriosis.

Authors - Xiaoshan Chai
humanWB,IP

Cell Death Dis

MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma

Authors - Jinrong Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Satyendra (Verified Customer) (07-16-2021)

I got really nice band of PRMT5 Knock out and knockdown

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293
PRMT5 Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:18436-1-AP)