PROX1 Monoclonal antibody

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  WB analysis of HuH-7 using 67438-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 67438-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67438-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67438-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HuH-7 using 67438-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (67438-1-Ig, Clone: 1D4B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

PROX1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67438-1-Ig

Clone No.1D4B1

Publications(2)

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1D4B1, Homeobox prospero-like protein PROX1, prospero homeobox 1, Prospero homeobox protein 1, PROX-1

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Tested Applications

• Positive WB detected in: HuH-7 cells, rat liver tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67438-1-Ig targets PROX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9681

  Product name: Recombinant human PROX1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 402-737 aa of BC024201

  Sequence: FHTANQRLQCFGDVIIPNPLDTFGNVQMASSTDQTEALPLVVRKNSSDQSASGPAAGGHHQPLHQSPLSATTGFTTSTFRHPFPLPLMAYPFQSPLGAPSGSFSGKDRASPESLDLTRDTTSLRTKMSSHHLSHHPCSPAHPPSTAEGLSLSLIKSECGDLQDMSEISPYSGSAMQEGLSPNHLKKAKLMFFYTRYPSSNMLKTYFSDVKFNRCITSQLIKWFSNFREFYYIQMEKYARQAINDGVTSTEELSITRDCELYRALNMHYNKANDFEVPERFLEVAQITLREFFNAIIAGKDVDPSWKKAIYKVICKLDSEVPEIFKSPNCLQELLHE

• Full Name: prospero homeobox 1
• Calculated Molecular Weight: 737 aa, 83 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC024201
• Gene Symbol: PROX1
• Gene ID (NCBI): 5629
• RRID: AB_2882674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92786
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
human	WB	J Transl Med; Metabolic expression profiling analysis reveals pyruvate-mediated EPHB2 upregulation promotes lymphatic metastasis in head and neck squamous cell carcinomas; Authors - Jingjing Miao