PRPS1 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 86217-1-RR (same clone as 86217-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86217-1-RR (PRPS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86217-1-PBS in a different storage buffer formulation.

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  Sample test of MP02295-1

  The mean PRPS1 concentration was determined to be 62.7 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02295-1

  Cytometric bead array standard curve of MP02295-1, PRPS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86217-2-PBS. Detection antibody: 86217-1-PBS. Standard: Ag7907. Range: 1.56-200 ng/mL.

PRPS1 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 86217-1-PBS

Clone No.250201C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

ARTS, CMTX5, EC:2.7.6.1, KIAA0967, PPRibP

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Product Information

86217-1-PBS targets PRPS1 as part of a matched antibody pair:

MP02295-1: 86217-2-PBS capture and 86217-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRPS1 fusion protein Ag7907
• Full Name: phosphoribosyl pyrophosphate synthetase 1
• Calculated Molecular Weight: 318 aa, 35 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC001605
• Gene Symbol: PRPS1
• Gene ID (NCBI): 5631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P60891
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRPS1, also named as Ribose-phosphate pyrophosphokinase 1, is a 318 amino acid protein, which belongs to the ribose-phosphate pyrophosphokinase family. PRPS1 can form Homodimer and the active form is probably a hexamer composed of 3 homodimers. PRPS1 catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) which is essential for nucleotide synthesis. PRPS1 as a module has an association with the Hepatocellular carcinoma.