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PTPN11 Monoclonal antibody

PTPN11 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3F8A8

Cat no : 66795-1-Ig

Synonyms

BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN11 Monoclonal antibody (66795-1-Ig)

    WB analysis using 66795-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66795-1-Ig (PTPN11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PTPN11 Monoclonal antibody (66795-1-Ig)

    WB analysis of HeLa using 66795-1-Ig

    WB result of PTPN11 antibody (66795-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN11 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PTPN11 Monoclonal antibody (66795-1-Ig)

    IHC staining of mouse testis using 66795-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PTPN11 Monoclonal antibody (66795-1-Ig)

    IHC staining of mouse testis using 66795-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PTPN11 Monoclonal antibody (66795-1-Ig)

    IF Staining of MCF-7 using 66795-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66795-1-Ig (PTPN11 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PTPN11 Monoclonal antibody (66795-1-Ig)

    IF Staining of MCF-7 using 66795-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11 antibody (66795-1-Ig, Clone: 3F8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using PTPN11 Monoclonal antibody (66795-1-Ig)

    FC experiment of MCF-7 using 66795-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PTPN11 (66795-1-Ig, Clone:3F8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66795-1-Ig targets PTPN11 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTPN11 fusion protein Ag13649
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC008692
Gene symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PTPN11 antibody 66795-1-IgDownload protocol
IHC protocol for PTPN11 antibody 66795-1-IgDownload protocol
IF protocol for PTPN11 antibody 66795-1-IgDownload protocol
FC protocol for PTPN11 antibody 66795-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols