SHP2 Monoclonal antibody, PBS Only

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  WB analysis using 66795-1-Ig (same clone as 66795-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66795-1-Ig (PTPN11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66795-1-Ig (same clone as 66795-1-PBS)

  WB result of PTPN11 antibody (66795-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN11 transfected HeLa cells. This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11/SHP2 antibody (66795-1-Ig, Clone: 3F8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11 antibody (66795-1-Ig, Clone: 3F8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PTPN11 (66795-1-Ig, Clone:3F8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

SHP2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66795-1-PBS

Clone No.3F8A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PTPN11/SHP2, PTPN11, BPTP 3, BPTP3, CFC

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Product Information

66795-1-PBS targets SHP2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13649

  Product name: Recombinant human PTPN11 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-357 aa of BC008692

  Sequence: MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTT

• Full Name: protein tyrosine phosphatase, non-receptor type 11
• Calculated Molecular Weight: 597 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC008692
• Gene Symbol: PTPN11
• Gene ID (NCBI): 5781
• RRID: AB_2882139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q06124
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.