PWWP2B Polyclonal antibody

• 

  WB analysis using 32112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32112-1-AP (PWWP2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

PWWP2B Polyclonal Antibody for WB, ELISA

Cat No. 32112-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PWWP Domain Containing 2, PWWP Domain Containing 2B , PWWP domain-containing protein 2B, PWWP2

---

Tested Applications

• Positive WB detected in: A549 cells, BxPC-3 cells, HaCaT cells, HeLa cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32112-1-AP targets PWWP2B in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PWWP2B fusion protein Ag36839
• Full Name: PWWP domain containing 2B
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC068574
• Gene Symbol: PWWP2B
• Gene ID (NCBI): 170394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6NUJ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PWWP2B (PWWP structural domain protein 2B) is a PWWP structural domain-containing protein involved in a variety of cellular functions and biological processes. PWWP2B is highly expressed in brown and beige adipose tissue and its expression is induced by cold stimulation. It has been shown that PWWP2B negatively regulates adipose tissue thermogenesis by recruiting and stabilizing the histone deacetylase HDAC1/2, which binds to the promoter regions of thermogenic genes and reduces histone acetylation levels. In adipocytes, PWWP2B acts as part of the nucleosome remodeling and deacetylation complex (NuRD) to inhibit the expression of thermogenic genes and avoid overactivation of thermogenic functions. In addition, PWWP2B plays an important role in DNA damage repair, as its PWWP structural domain moves to DNA damage sites, interacts with UHRF1 and recruits MRE11 to promote DNA end resection and homologous recombination repair. In pathology, downregulation of PWWP2B is associated with recurrent gastric cancer and promotes cell proliferation and migration.