PYGM-Specific Polyclonal antibody, PBS Only

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  WB analysis of HepG2 using 19716-1-AP (same clone as 19716-1-PBS)

  WB result of PYGM-Specific antibody (19716-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGM-Specific transfected HepG2 cells. This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

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  WB analysis using 19716-1-AP (same clone as 19716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19716-1-AP (PYGM-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 19716-1-AP (same clone as 19716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 19716-1-AP (same clone as 19716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 19716-1-AP (same clone as 19716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 19716-1-AP (same clone as 19716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19716-1-AP (same clone as 19716-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PYGM-Specific antibody (19716-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PYGM-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 19716-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

19716-1-PBS targets PYGM-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphorylase, glycogen, muscle
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97-105 kDa
• GenBank Accession Number: NM_005609
• Gene Symbol: PYGM
• Gene ID (NCBI): 5837
• RRID: AB_10642009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11217
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PYGM belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) which also known as McArdle disease. The antibody is specific to PYGM.