PYROXD1 Recombinant antibody

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  WB analysis of HeLa using 84289-7-RR

  WB result of PYROXD1 antibody (84289-7-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYROXD1 transfected HeLa cells.

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  WB analysis using 84289-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84289-7-RR (PYROXD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84289-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84289-7-RR against Human PYROXD1 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

PYROXD1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84289-7-RR

Clone No.241639D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

241639D7, pyridine nucleotide disulphide oxidoreductase domain 1

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Tested Applications

• Positive WB detected in: HeLa cells, U-87 MG cells, Caco-2 cells, COLO 320 cells, PC-3 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84289-7-RR targets PYROXD1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PYROXD1 fusion protein Ag34768
• Full Name: pyridine nucleotide-disulphide oxidoreductase domain 1
• Observed Molecular Weight: 55~60 kDa
• GenBank Accession Number: BC021662
• Gene Symbol: PYROXD1
• Gene ID (NCBI): 79912
• RRID: AB_3671834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8WU10
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

pyridine nucleotide-disulfide oxidoreductase domain 1 (PYROXD1) is a putative oxidoreductase that co-evolved with the tRNA-LC and is essential for its activity in cells and in vivo. PYROXD1 oxidizes NAD(P)H to NAD(P)+ with tightly controlled kinetics, reducing one molecule of O2 into H2O2 per turnover. PYROXD1 is expressed across a range of tissue types in humans, including skeletal muscle, and is conserved across many species.