Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant antibody

Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB

Conjugate

Unconjugated

CloneNo.

1P6

Cat no : 80093-1-RR

Synonyms

DKFZp686A2068, KRS2, MST 1, MST1, MST1 (T183), MST1 (T183)/MST2(Thr180), MST1 T183, MST1 Thr183, MST2 T180, MST2 Thr180, MST2(Thr180), Phospho-MST1 (Thr183)/MST2 (Thr180), Phospho-MST1 (Thr183)/MST2(Thr180), serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, Calyculin A treated HeLa cells, Staurosporine treated Ramos cells, Staurosporine treated Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

80093-1-RR targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name serine/threonine kinase 4
Calculated molecular weight 56 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC005231
Gene symbol STK4
Gene ID (NCBI) 6789
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

Protocols

Product Specific Protocols
WB protocol for Phospho-MST1 (Thr183)/MST2 (Thr180) antibody 80093-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

Role of the hippo signaling pathway in the extracellular matrix degradation of chondrocytes induced by fluoride exposure

Authors - Fang-fang Yu