Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant antibody

Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1P6

Cat no : 80093-1-RR

Synonyms

DKFZp686A2068, KRS2, MST 1, MST1, MST1 (T183), MST1 (T183)/MST2(Thr180), MST1 T183, MST1 Thr183, MST2 T180, MST2 Thr180, MST2(Thr180), Phospho-MST1 (Thr183)/MST2(Thr180), serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, Calyculin A treated HeLa cells, Staurosporine treated Ramos cells, Staurosporine treated Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

80093-1-RR targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name serine/threonine kinase 4
Calculated molecular weight 56 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC005231
Gene symbol STK4
Gene ID (NCBI) 6789
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors.Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

Protocols

Product Specific Protocols
WB protocol for Phospho-MST1 (Thr183)/MST2 (Thr180) antibody 80093-1-RRDownload protocol
Standard Protocols
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