Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody

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  WB analysis using 60597-1-Ig

  Non-treated cells and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60597-1-Ig

  Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60597-1-Ig

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Phospho-PERK/EIF2AK3 (Ser719) Monoclonal Antibody for WB, ELISA

Cat No. 60597-1-Ig

Clone No.3H7A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

EIF2AK3, PERK, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3, EIF2AK 3

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, PC-3 cells, Jurkat cells, Calyculin A treated Jurkat cells, Calyculin A treated HEK-293 cells, Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60597-1-Ig targets Phospho-PERK/EIF2AK3 (Ser719) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Calculated Molecular Weight: 1116 aa, 125 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC126354
• Gene Symbol: PERK
• Gene ID (NCBI): 9451
• RRID: AB_3670269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NZJ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)