Phospho-STK11/LKB1 (Thr189) Recombinant antibody

Phospho-STK11/LKB1 (Thr189) Recombinant Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5J9

Cat no : 80127-1-RR

Synonyms

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11



Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, NIH/3T3 cells, Calyculin A treated HEK-293 cells, Calyculin A treated PC-3 cells, Calyculin A treated NIH/3T3 cells
Positive IF detected inCalyculin A treated PC-3 cells
Positive FC detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

80127-1-RR targets Phospho-STK11/LKB1 (Thr189) in WB, IF, FC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name serine/threonine kinase 11
Calculated molecular weight 49 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC007981
Gene symbol STK11
Gene ID (NCBI) 6794
RRIDAB_2918867
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.

Protocols

Product Specific Protocols
WB protocol for Phospho-STK11/LKB1 (Thr189) antibody 80127-1-RRDownload protocol
IF protocol for Phospho-STK11/LKB1 (Thr189) antibody 80127-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols