Phospho-STK11/LKB1 (Thr189) Recombinant antibody, PBS Only

Phospho-STK11/LKB1 (Thr189) Recombinant Antibody for WB, IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5J9

Cat no : 80127-1-PBS

Synonyms

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11

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Validation Data Gallery

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  WB analysis using 80127-1-RR (same clone as 80127-1-PBS)

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80127-1-RR (same clone as 80127-1-PBS)

  Non-treated NIH/3T3 and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80127-1-RR (same clone as 80127-1-PBS)

  Non-treated HEK-293 and Calyculin A treated HEK-293 cells subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 80127-1-RR (same clone as 80127-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using Phospho-STK11/LKB1 (Thr189) antibody (80127-1-RR, Clone: 5J9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 80127-1-RR (same clone as 80127-1-PBS)

  1X10^6 PC-3 cells untreated (dashed lines) or Calyculin A treated (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-STK11 (Thr189) (80127-1-RR, Clone:5J9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80127-1-PBS targets Phospho-STK11/LKB1 (Thr189) in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: serine/threonine kinase 11
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC007981
• Gene Symbol: STK11
• Gene ID (NCBI): 6794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.