Tested Applications
| Positive WB detected in | UV treated HEK-293T cells, LPS treated THP-1 cells, UV treated NIH/3T3 cells |
| Positive IF/ICC detected in | LPS treated THP-1 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28796-1-AP targets Phospho-p38 MAPK (Thr180/Tyr182) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase 14 |
| Calculated Molecular Weight | 360 aa, 41 kDa |
| Observed Molecular Weight | 38-42 kDa |
| GenBank Accession Number | BC031574 |
| Gene Symbol | p38 MAPK |
| Gene ID (NCBI) | 1432 |
| RRID | AB_2918205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16539 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-p38 MAPK (Thr180/Tyr182) antibody 28796-1-AP | Download protocol |
| WB protocol for Phospho-p38 MAPK (Thr180/Tyr182) antibody 28796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibody (Cat# 28796-1-AP) has 572 total citations. Citations appear in journals including Molecular Cancer, Nature Aging, ACS Nano, Advanced Science, Cell Reports, Journal of Ethnopharmacology, Journal of Translational Medicine, Free Radical Biology and Medicine, and Journal of Nanobiotechnology. Research fields span oncology, neuroinflammation, cardiovascular disease, fibrosis, ferroptosis, immunology, traditional Chinese medicine, and MAPK/NF-κB signaling pathway studies, primarily using Western blot.
| Species | Application | Title |
|---|---|---|
Mol Cancer Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity | ||
Mil Med Res D-mannose alleviates intervertebral disc degeneration through glutamine metabolism | ||
Cancer Commun (Lond) PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism. | ||
Cancer Commun (Lond) Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma | ||
Nat Aging Senescent macrophages induce ferroptosis in skeletal muscle and accelerate osteoarthritis-related muscle atrophy | ||
Cell Mol Immunol Cathepsin C orchestrates RSV-induced asthma exacerbation through the dual effect of monocyte-derived macrophages. |
