Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibody

Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (3)

Applications

WB,IF,ELISA

Conjugate

Unconjugated

Cat no : 28796-1-AP

Synonyms

CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38ALPHA, Phospho-p38 MAPK (Thr180/Tyr182), PRKM14, PRKM15, RK, SAPK2A



Tested Applications

Positive WB detected inUV treated HEK-293T cells, LPS treated THP-1 cells, UV treated NIH/3T3 cells
Positive IF detected inLPS treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28796-1-AP targets Phospho-p38 MAPK (Thr180/Tyr182) in WB,IF,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase 14
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight38-42 kDa
GenBank Accession NumberBC031574
Gene Symbol MAPK14
Gene ID (NCBI) 1432
RRIDAB_2918205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)

Protocols

Product Specific Protocols
WB protocol for Phospho-p38 MAPK (Thr180/Tyr182) antibody 28796-1-APDownload protocol
IF protocol for Phospho-p38 MAPK (Thr180/Tyr182) antibody 28796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Authors - Boying Zhao
humanWB

Cell Death Dis

USP4 promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting TAK1

Authors - Hongdian Zhang
humanWB

Clin Transl Med

CircRNA DDX21 acts as a prognostic factor and sponge of miR-1264/QKI axis to weaken the progression of triple-negative breast cancer.

Authors - Renhong Huang
mouseWB

J Transl Med

Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways

Authors - Xin-Sen Chen
mouseWB

Int J Nanomedicine

The Immune-Enhancing Properties of Hwanglyeonhaedok-Tang-Mediated Biosynthesized Gold Nanoparticles in Macrophages and Splenocytes.

Authors - Xiao-Jie Mi
pigWB

Int J Mol Sci

circBTBD7 Promotes Immature Porcine Sertoli Cell Growth through Modulating miR-24-3p/ MAPK7 Axis to Inactivate p38 MAPK Signaling Pathway

Authors - Qiao Bian