Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibody

Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (4)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28796-1-AP

Synonyms

CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38ALPHA, Phospho-p38 MAPK (Thr180/Tyr182), PRKM14, PRKM15, RK, SAPK2A



Tested Applications

Positive WB detected inUV treated HEK-293T cells, LPS treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 26 publications below

Product Information

28796-1-AP targets Phospho-p38 MAPK (Thr180/Tyr182) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase 14
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight38-42 kDa
GenBank accession numberBC031574
Gene symbol MAPK14
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)

Protocols

Product Specific Protocols
WB protocol for Phospho-p38 MAPK (Thr180/Tyr182) antibody 28796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Chem Biol Interact

In addition to its endosomal escape effect, platycodin D also synergizes with ribosomal inactivation protein to induce apoptosis in hepatoma cells through AKT and MAPK signaling pathways.

Authors - Jin Lei
mouseWB

ACS Chem Neurosci

Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus

Authors - Yan Sun
pigWB

Int J Mol Sci

circBTBD7 Promotes Immature Porcine Sertoli Cell Growth through Modulating miR-24-3p/ MAPK7 Axis to Inactivate p38 MAPK Signaling Pathway

Authors - Qiao Bian
mouseWB

Int J Nanomedicine

The Immune-Enhancing Properties of Hwanglyeonhaedok-Tang-Mediated Biosynthesized Gold Nanoparticles in Macrophages and Splenocytes.

Authors - Xiao-Jie Mi
mouseWB

World J Gastrointest Oncol

Frankincense myrrh attenuates hepatocellular carcinoma by regulating tumor blood vessel development through multiple epidermal growth factor receptor-mediated signaling pathways.

Authors - Piao Zheng
mouseWB

J Vet Res

Estrogen-related Receptor α (ERRα) Functions in The Hypoxic Injury of Microglial Cells.

Authors - Chao-Yang Deng