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Prestained Protein Marker

Molecular Weight

10-180 kDa

Detection Method

Colorimetric

Number of Markers

10

Stain Type

3 colors: Blue, Red, Green

Reviews (19)

Cat no : PL00001

Validation Data Gallery

  • SDS-PAGE band profile of Prestained Protein Ladder

    SDS-PAGE band profile of Prestained Protein Ladder

    Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

  • Migration patterns of Prestained Protein Ladder in different electrophoretic conditions

    Migration patterns of Prestained Protein Ladder in different electrophoretic conditions

    Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in Figure 2.

Product Information

The PL00001 Regular Range Protein Marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins.

Detection Method Colorimetric
Molecular Weight ~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180 kDa
Number of Markers 10
Size Range 10 to 180
Stain Type 3 colors: Blue, Red, Green
Contents Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage Store product at 4 C for up to 12 weeks. For longer storage, aliquot and store at -20 C for up to 1 year.
Instructions The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3 -5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Reviews

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