Prestained Protein Marker
Molecular Weight
10-180 kDa
Detection Method
Colorimetric
Number of Markers
10
Stain Type
3 colors: Blue, Red, Green
Reviews (19)
Cat no : PL00001
Validation Data Gallery
Product Information
The PL00001 Regular Range Protein Marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins.
Detection Method | Colorimetric |
Molecular Weight | ~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180 kDa |
Number of Markers | 10 |
Size Range | 10 to 180 |
Stain Type | 3 colors: Blue, Red, Green |
Contents | Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). |
Storage | Store product at 4 C for up to 12 weeks. For longer storage, aliquot and store at -20 C for up to 1 year. |
Instructions | The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3 -5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading. |
Reviews
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