Prestained protein marker
Molecular Weight
10-180 kDa
Detection Method
Colorimetric
Number of Markers
10
Stain Type
3 colors: Blue, Red, Green
Cat no : PL00001
"Prestained protein marker" Comparison
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Product Information
The PL00001 Regular Range Protein Marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage
Store product at 4℃ for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.
Usage
The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.
Detection Method
Colorimetric
Molecular Weight
~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180 kDa
Number of Markers
10
Size Range
10 to 180
Stain Type
3 colors: Blue, Red, Green
Cited in Article as
PL00001, Prestained protein marker, Proteintech, IL, USA
Documentation
| SDS |
|---|
| Prestained Potein Marker 10-180kDa |
Publications
| Application | Title |
|---|---|
Front Immunol Roles of RAGE/ROCK1 Pathway in HMGB1-Induced Early Changes in Barrier Permeability of Human Pulmonary Microvascular Endothelial Cell. | |
J Ethnopharmacol The mechanisms of action of WeiChang'An Pill (WCAP) treat diarrhoea-predominant irritable bowel syndrome (IBS-D) using network pharmacology approach and in vivo studies. | |
Naunyn Schmiedebergs Arch Pharmacol Aconitine linoleate, a natural lipo-diterpenoid alkaloid, stimulates anti-proliferative activity reversing doxorubicin resistance in MCF-7/ADR breast cancer cells as a selective topoisomerase IIα inhibitor. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christine (Verified Customer) (10-27-2022) | Very good coloured protein ladder. I loaded 1.5 ul on a SDS gel PAGE and transferred to nitrocellulose membrane and the ladder was extremely clean and visible. I will load less next time, 1 ul should be more than enough
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FH Silvia (Verified Customer) (10-18-2022) | not equal as other used but ok
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FH Agnieszka (Verified Customer) (10-14-2022) | Very versatile ladder! I do enjoy the quantity and positioning of the bands, especially under 50kDa. This ladder allowed me to assign MW to proteins which normally fall between the bands
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FH Filippo (Verified Customer) (09-09-2022) | Very good prestained protein marker. Clear bands and wide range.
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FH Irina (Verified Customer) (09-06-2022) | This prestained protein ladder works really good in 10-180kDa range
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FH Hasan (Verified Customer) (09-02-2022) | The samples were run on a 15% SDS-PAGE
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FH FIRUZE (Verified Customer) (08-26-2022) | It works very well!
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FH Eleftheria (Verified Customer) (08-26-2022) | Accurate MW range, great staining
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FH Georgina (Verified Customer) (07-04-2022) | Great protein marker! Makes cutting membranes very easy.
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FH Debarpan (Verified Customer) (06-09-2022) | I found the product really promising. I compared it to PageRuler that we use traditionally and it matches the results quite perfectly.
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FH James (Verified Customer) (05-24-2022) | Great protein ladder. Works exactly as expected :)
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FH Lucia (Verified Customer) (05-12-2022) | I used it and it worked very well, I recommend it
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FH Samuele (Verified Customer) (05-04-2022) | Reliable marker with good sharps bands and good signal even with low quantity
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FH Lisa (Verified Customer) (05-01-2022) | It works very well giving sharp and reliable bands
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FH Dalila (Verified Customer) (04-12-2022) | The marker works very good. It was easy to distinguish different bands. I used it to look at my protein of interest and house keeping, kDA bands looks very good.
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FH Irene (Verified Customer) (04-12-2022) | Perfect! It does its job. I've used it for a western blot. I recommended it.
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FH Matteo (Verified Customer) (04-11-2022) | Used for WB, worked perfectly fine, also compared with my previous protein marker. Totally recommend
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FH Laura (Verified Customer) (04-08-2022) | Great protein ladder. Sharp bands and bright colors after transfer to nitrocellulose membrane. It comes in ready-to-use format, which is quite convenient.
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FH Brogan (Verified Customer) (04-05-2022) | very clear ladder, easy to see on PVDF membrane. Worth the price.
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FH Laura (Verified Customer) (04-01-2022) | The ladder works very well. I’ve used it to look at p53 isoforms. Good colors.
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FH AVIKA (Verified Customer) (03-19-2022) | The protein ladder worked well and the 3 different colors made the detection easier.
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FH Carlo (Verified Customer) (03-16-2022) | The protein ladder is well made and mantains color for long time
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FH camilla (Verified Customer) (03-16-2022) | i tried this as part of the phd free trial kit and i'm very satisfied of the result, i'm considering ordering this again
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FH Victoria (Verified Customer) (03-12-2022) | Clear, bright bands. Nicely colour coded for ease of use.
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FH Oliviero (Verified Customer) (03-11-2022) | Prestained proteine marker works very well in a Wb gel (1mm) with a persistent color also After washing
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FH massimo (Verified Customer) (03-10-2022) | excellent protein markers
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FH Vincenza (Verified Customer) (03-01-2022) | The marker works really well, it is precise and bright. Excellent product. Recommended.
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FH Angelo (Verified Customer) (02-28-2022) | Great Ladder very clean and precise, I used 5uL and the color on an homemade 12% gel and the resolution it has been perfect
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FH Laura (Verified Customer) (02-25-2022) | Very defined bands
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FH Giorgia (Verified Customer) (02-25-2022) | Markers ran well and the color scale was clearly visible
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FH Cristina (Verified Customer) (02-25-2022) | Markers worked very well, with bands well defined
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FH Abigail (Verified Customer) (02-22-2022) | Ladder separated out well, colours made it really easy to read. I really like this ladder!
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FH Alberto (Verified Customer) (02-15-2022) | First time trial. Positive impression. The ladder is just comparable to the one we normally use (other brand). It was suggested to load 3-5uL but personally I would use 10uL for a more definite ladder. No complaints.
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FH Loic (Verified Customer) (02-15-2022) | Worked great for Western Blot!
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FH Alessandro (Verified Customer) (01-28-2022) | The prestained protein marker is well visibile and the color brightness is conserved in time
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FH Federica (Verified Customer) (01-28-2022) | prestained protein marker maintains colour brightness even after strippimng procedures.
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FH Kayleigh (Verified Customer) (01-27-2022) | good ladder, transfers well
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FH Alessandro (Verified Customer) (12-17-2021) | Worked out great, nice band separation
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FH Luke (Verified Customer) (11-24-2021) | Ladder ran really nicely and gave clear and well defined bands on my gel
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FH Andrew (Verified Customer) (11-15-2021) | Worked well.
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FH Kai (Verified Customer) (11-05-2021) | The prestained protein ladder shows clear bands for a reasonable range of molecular weights. Including bands for smaller proteins might be useful for some cases.
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FH Clara (Verified Customer) (10-15-2021) | Protein ladder used for SDS PAGE 10%, minigel, 1mm thikness. 10ul of ladder were loaded after running the gel the bands higher then the 70 kDA band disappeared
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FH Macarena (Verified Customer) (08-23-2021) | Very nice ladder. I tried using only 2uL and the bands were still clear.
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FH Maria (Verified Customer) (08-23-2021) | Cell whole lysate labelled with No-Stain (20 ug protein), VWR VI marker 9 uL, Proteintech PL00001 5 uL.
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FH Kishore (Verified Customer) (07-20-2021) | Perfect marker for protein gels, crispy bands
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FH Tatiana (Verified Customer) (07-15-2021) | Very good ladder. The bands are well separated and visible.
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FH Vasudha (Verified Customer) (07-01-2021) | The bands are nicely separated and colorful.I used 5ul but looks like 2ul would be sufficient to see the bands.
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FH Lucija (Verified Customer) (06-29-2021) | I requested a sample of Prestained protein marker 10-180 kDa) and received it in less than a week. The product is amazing, after loading only 3 uL (as I usually do), SDS-PAGE and transfer to membrane resulted in sharp and bright bands. I highly recommend this product.
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FH PRADEEP (Verified Customer) (06-27-2021) | Excellent Prestained Protein Marker
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FH Daniel (Verified Customer) (06-11-2021) | Works exactly as you'd expect it to, no complaints. Colours are vibrant and clear. Used in SDS-PAGE. This is a higher concentration than I'm used to (2-5x more concentrated at least), you only need to use 1-3 µL in a 50µL well
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FH Yehuda (Verified Customer) (05-31-2021) | Great prestained protein ladder with clear colourful bands. Good separation in SDS-PAGE and nice transfer.
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FH Zoe (Verified Customer) (04-22-2021) | Really clear protein ladder that ran well, colours help visualisation on SDS-PAGE gel
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FH Lucy (Verified Customer) (04-19-2021) | Nice ladder, good seperation
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FH Jessica (Verified Customer) (04-13-2021) | The protein ladder worked comparably well to the ladder that we currently use from BioRad. Unfortunately, the BioRad ladder is cheaper to buy in bulk but I wouldn't hesitate to use this one.
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FH Elizabeth (Verified Customer) (04-12-2021) | in lane on the right, tested against precision plus protein dual color standard from Bio-Rad, cat# 1610374 (left). red and green channel fluorescence in black and white included, ladder looked much better in the red channel.
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FH Ivan (Verified Customer) (04-12-2021) | Fantastic Protein Marker. Highly recommendable for any use: gels, blotting, etc. etc. etc. Clear colourful bands.
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FH T (Verified Customer) (04-08-2021) | Great protein marker, easy to use. It does the job!
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FH Brandon (Verified Customer) (03-30-2021) | The marker is easy to use and the bands are nicely separated.
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FH Ian (Verified Customer) (03-28-2021) | Product reacted well to the experiment and yielded good results.
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FH Javier (Verified Customer) (03-25-2021) | Ladder separates and aligns great my known protein.
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FH Elisa (Verified Customer) (03-19-2021) | I compared with other company ladder and looks pretty good, with only 4uL. Photo form a 4-20% TGX gel
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FH XIAOBING (Verified Customer) (03-02-2021) | this product works great.
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FH Julie (Verified Customer) (03-02-2021) | I like this new ladder. Just have some difficulties to observe the green ladder during migration but become better after transfer.
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FH Pradeep (Verified Customer) (02-18-2021) | It is great.. worked well on SDS-PAGE
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FH Hala (Verified Customer) (02-08-2021) | very good working
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FH Tom (Verified Customer) (01-19-2021) | 3ul of ladder loaded onto 12% acrlyamide gel. Transfer to nitrocellulose membrane and stained with ponceau to assess total protein, as seen in image.Ladder was clearly visible in both the gel and the membrane.
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FH Petra (Verified Customer) (01-12-2021) | Works well both ín SDS-polyacrylamide gel and on PVDF membranes. Load 1 ul and still clearly visible.
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FH Tom (Verified Customer) (01-08-2021) | Loaded 5uL of protein ladder into left lane on 12.5% gel, followed by transfer to nitrocellulose membrane.Ladder was clearly visible in both gel and membrane. Image captured after ponceau stain.
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FH Malak (Verified Customer) (12-28-2020) | I used this marker with Biorad precasted gels 4-15% and it separates perfectly allowing you to compare the size of your protein in a pretty accurate way. I would definitely order it for next experiments.
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FH Dorina (Verified Customer) (12-23-2020) | I have used this ladder a lot and it always worked reliably and consitent and I never had any issues. The bands show up strong and clearly as expected
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FH Kalin (Verified Customer) (12-21-2020) | It is great.
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FH Sheetal (Verified Customer) (12-14-2020) | This protein ladder was working great, it has a single reference band which avoid confusions of comparing between two or three reference bands. And it runs the same way as shown in datasheet if we run the marker in different percentages of gels.
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FH Lori (Verified Customer) (11-24-2020) | I wanted to test out 2,2,2-trichloroethanol in my resolving gel and it was good so I asked if the student would like to continue on with procedure and transfer to blot they did not have time so we did not go further than the gel electrophoresis.The ladder was bright and you could see the three distinct colors of the various bands, blue, green and red. I would definitely continue to use this product in my western blot analyses
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FH Lobarkhon (Verified Customer) (11-04-2020) | Protein Ladder works very well. I have used 4 - 5ul and both band clearly visible.With different colors easy to measure the weight of sample protein.
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FH Tamara (Verified Customer) (10-29-2020) | Good staining and good bands quality
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FH Ming Yin (Verified Customer) (10-19-2020) | The ladder worked perfectly. There were no differences in performance between this ladder and previous ladder I have used. Coloured markers were nice for images.
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FH Emanuela (Verified Customer) (09-29-2020) | The ladder runs well in SDS gel loading 2,5 ul, the bands match well with the tested antibodies (actin, sigma1r, VDAC, IP3R, catalase)
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FH Satyendra (Verified Customer) (09-28-2020) | I used the protein ladder and it works fine for me
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FH Bryce (Verified Customer) (09-18-2020) | This multicolored protein ladder was easy to see with only 5 uL loaded into the well.
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FH Jie (Verified Customer) (09-01-2020) | Work well on SDS-PAGE
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FH Sarah (Verified Customer) (08-28-2020) | Good!
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FH Janardhan (Verified Customer) (08-04-2020) | I used 5ul, the bands are clearly visible and the color bands are helpful to detect the molecular weight easily
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FH Nayden (Verified Customer) (07-21-2020) | Gives clear bands on polyacrylamide gel, and on nitrocellulose membrane after standard 1h wet transfer.
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FH Onyekachim (Verified Customer) (07-21-2020) | The ladder ran as expected and was easy to detect on the gel.
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FH Teresa (Verified Customer) (07-20-2020) | Prestained protein marker can be used 4 ul per lane. I saw bright, robust bands on my PVDF that lasted through 3 rounds of antibody incubations and ECL.
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FH pitna (Verified Customer) (07-16-2020) | 1 lane : predestined protein marker from proteintech (5ul)2 lane : protein marker from lab (10ul)3, 4 lane : 5ug, 10ug Postmortem brain tissue
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FH Boglarka (Verified Customer) (07-14-2020) | It is easily visible and the color-coded bands are really helpful.
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FH Trisiani (Verified Customer) (07-09-2020) | The markers look nice and transferred well for Western blot.
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FH Aarthi (Verified Customer) (07-06-2020) | I needed a ladder with a 75 kDa band. The proteintech ladder worked very well for my purpose.
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FH Rakesh (Verified Customer) (07-06-2020) | The Ladder resolves very well on 12% SDS-PAGE gel. It transfers very efficiently onto the PVDF membrane upon Western transfer. The ladder is retained on the blot even after stripping the blot. However, upon stripping the green band of the ladder disappears.
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FH deng (Verified Customer) (07-06-2020) | I recently used the protein ladder and am happy to say it works as same as product from Bio Rad dual color standards.
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FH Kunny (Verified Customer) (07-06-2020) | Works great
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FH Eya (Verified Customer) (07-02-2020) | I tested this product in a wb gel at 10%, the bands were clearly visible and clear
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FH Carlos (Verified Customer) (03-18-2020) | The ladder worked beautifully in our hands. The range of weights is very suitable for our studies to try fing new targets for anti-epileptic drugs
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FH Phoebe (Verified Customer) (03-18-2020) | I have used this as a replacement for PageRuler Plus for a western with a large (~200kDa) protein, and it worked brilliantly.
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FH Lorraine (Verified Customer) (03-17-2020) | Worked perfectly well and will definitely consider ordering more
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FH Alejandra (Verified Customer) (03-12-2020) | Separetion of the bands was nice, th green color of the ~23kDa band was a bit less visible the other 2 colours but it was ok.
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FH Kamila (Verified Customer) (03-11-2020) | I've used the Pritein Ladder in several Western Blot analysis and I can now say, that the bands are very clearly visible on gel and on the membrane, what makes the protein identification faster and cutting membranes easier.
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FH Stephen (Verified Customer) (03-09-2020) | 5 micro L of ladder loaded on protein 4-15% tris glycine gel . Gel wet transferred onto 0.22um nitrocellulose membrane . Membrane was stained with amido black and destained with 20% methanol 10% acetic acid Protein marker is still visible with c colours bands as well still good product
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FH Petra (Verified Customer) (03-09-2020) | The ladder ran and separated well on the gel. I compared it to my normal protein ladder from ThermoFisher.I didn't image it in the end, since my actual samples didn't work that time!
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FH Sibylle (Verified Customer) (03-08-2020) | Molecular weight ladder worked well overall, when we loaded 5ul of the pre-stained ladder. However, the high molecular bands (140-180kDa) were very faint, and then completely disappeared after the PVDF membranes were stripped to re-probe for a second marker.
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FH Miray (Verified Customer) (03-05-2020) | it works well for Western blot
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FH Anna (Verified Customer) (03-02-2020) | Very useful. I used only 3 ul and yet it looked great on the gel. I was able to follow the run and separate well the molecular weights of my interest. Having different colors makes it very easy to read.
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FH ALBERTO (Verified Customer) (03-02-2020) | The ladder works well, detecting the bands at the expected size
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FH Sara (Verified Customer) (03-02-2020) | Nice ladder! Very easy to see, both in the gel (while running and before transfer) and in the membrane (after transfer). The transfer from the gel to membrane was almost 100%.
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FH Veronica (Verified Customer) (03-02-2020) | A good protein ladder
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FH Anton (Verified Customer) (02-26-2020) | Good ladder run on SDS-PAGE gel. Thanks!
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FH Laura (Verified Customer) (02-25-2020) | I liked this product compared to another one I was using before because of the 3 colors it uses.
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FH Nicky (Verified Customer) (02-25-2020) | Excellent ladder, I liked it better than the one we currently use (SeeBlue Plus2 ladder) as the larger bands are more easily visible.
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FH David (Verified Customer) (02-24-2020) | Ladder worked really well, very clear both on the gel and the membrane after transfer.
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FH Deirdra (Verified Customer) (02-03-2020) | Great protein ladderClear running and the three different colours make it easy to distinguish different weights for antibody probing/ cutting membrane
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FH Serat-E (Verified Customer) (02-03-2020) | Great ladder, clear and useful. Small amount needed.
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