Prestained Protein Marker

Molecular Weight

10-180 kDa

Detection Method

Colorimetric

Number of Markers

10

Stain Type

3 colors: Blue, Red, Green

Reviews (19)

Cat no : PL00001

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Validation Data Gallery

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  SDS-PAGE band profile of Prestained Protein Ladder

  Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

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  Protein ladder tested with iBright

  4ul protein ladders were loaded in the gel with concentration 8%-18%. After Electrophoresis, the protein on the gel were transformed into the PVDF membrane. This image was taken by the iBright.

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  Migration patterns of Prestained Protein Ladder in different electrophoretic conditions

  Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in Figure 2.

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Product Information

The PL00001 Regular Range Protein Marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins.

• Detection Method: Colorimetric
• Molecular Weight: ~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180 kDa
• Number of Markers: 10
• Size Range: 10 to 180
• Stain Type: 3 colors: Blue, Red, Green
• Contents: Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
• Storage: Store product at 4 C for up to 12 weeks. For longer storage, aliquot and store at -20 C for up to 1 year.
• Instructions: The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3 -5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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