Prestained Protein Marker
Number of Markers
3 colors: Blue, Red, Green
Cat no : PL00001
The PL00001 Regular Range Protein Marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins.
|Molecular Weight||~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180 kDa|
|Number of Markers||10|
|Size Range||10 to 180|
|Stain Type||3 colors: Blue, Red, Green|
|Contents||Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).|
|Storage||Store product at 4 C for up to 12 weeks. For longer storage, aliquot and store at -20 C for up to 1 year.|
|Instructions||The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3 -5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.|
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