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Prestained protein marker

Molecular Weight

10-180 kDa

Detection Method

Colorimetric

Number of Markers

10

Stain Type

3 colors: Blue, Red, Green

Cat no : PL00001

Validation Data Gallery

  • Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

    Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

  • 4ul protein ladders were loaded in the gel with concentration 8%-18%. After Electrophoresis, the protein on the gel were transformed into the PVDF membrane. This image was taken by the iBright

    4ul protein ladders were loaded in the gel with concentration 8%-18%. After Electrophoresis, the protein on the gel were transformed into the PVDF membrane. This image was taken by the iBright

  • Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in Figure 2.

    Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in Figure 2.

Product Information

The PL00001 Regular Range Protein Marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins.

Publications

ApplicationTitle
WB

Naunyn Schmiedebergs Arch Pharmacol

Aconitine linoleate, a natural lipo-diterpenoid alkaloid, stimulates anti-proliferative activity reversing doxorubicin resistance in MCF-7/ADR breast cancer cells as a selective topoisomerase IIα inhibitor.

Authors - Shangxian Luan
View Article

J Ethnopharmacol

The mechanisms of action of WeiChang'An Pill (WCAP) treat diarrhoea-predominant irritable bowel syndrome (IBS-D) using network pharmacology approach and in vivo studies.

Authors - Yan Chen
View Article

Front Immunol

Roles of RAGE/ROCK1 Pathway in HMGB1-Induced Early Changes in Barrier Permeability of Human Pulmonary Microvascular Endothelial Cell.

Authors - Meng-Jiao Zhao
View Article