Prolactin Recombinant antibody

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  WB analysis using 85042-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85042-1-RR (Prolactin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85042-1-RR against Mouse Prolactin were performed. The affinity constant is 4.50 nM.

Prolactin Recombinant Antibody for WB, ELISA

Cat No. 85042-1-RR

Clone No.241771A5

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

241771A5

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat lung tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85042-1-RR targets Prolactin in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: prolactin
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NP_035294.2
• Gene Symbol: Prl
• Gene ID (NCBI): 19109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prolactin is also named as PRL and belongs to the somatotropin/prolactin family. The proteins encoded by PRL are secreted into the cell surroundings. And they are abundantly expressed in pituitary gland, adenohypophysis, decidua and testis. Indeed, chemically, prolactin appears in a multiplicity of posttranslational forms ranging from size variants to chemical modifications such as phosphorylation or glycosylation. It is not only synthesized in the pituitary gland, as originally described, but also within the central nervous system, the immune system, the uterus and its associated tissues of conception, and even the mammary gland itself (PMID: 11015620). Prolactin acts primarily on the mammary gland by promoting lactation (PMID: 30546056).