• Featured Product
  • KD/KO Validated

RAB3IP/Rabin8 Polyclonal antibody

RAB3IP/Rabin8 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12321-1-AP

Synonyms

Rab 3A interacting protein, Rab3A interacting protein, RAB3IP, Rabin 3, RABIN3, Rabin8, SSX2 interacting protein



Tested Applications

Positive WB detected inhuman brain tissue, human heart tissue, mouse heart tissue, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12321-1-AP targets RAB3IP/Rabin8 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity canine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB3IP/Rabin8 fusion protein Ag2974
Full Name RAB3A interacting protein (rabin3)
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC015548
Gene symbol RAB3IP
Gene ID (NCBI) 117177
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles.

Protocols

Product Specific Protocols
WB protocol for RAB3IP/Rabin8 antibody 12321-1-APDownload protocol
IHC protocol for RAB3IP/Rabin8 antibody 12321-1-APDownload protocol
IF protocol for RAB3IP/Rabin8 antibody 12321-1-APDownload protocol
IP protocol for RAB3IP/Rabin8 antibody 12321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

Cytoplasmic control of Rab family small GTPases through BAG6.

Authors - Toshiki Takahashi
  • KD Validated
humanWB

Small GTPases

Guanine nucleotide exchange factors activate Rab8a for Toll-like receptor signalling.

Authors - Samuel J Tong
  • KD Validated
mouseIF,IP

J Biol Chem

Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.

Authors - Tao Tao
humanWB, IP

Acta Naturae

Rabin8 Protein Interacts with GTPase Rheb and Inhibits Phosphorylation of Ser235/Ser236 in Small Ribosomal Subunit Protein S6.

Authors - Parkhitko Capital A Cyrillic А CA
humanWB

J Biol Chem

The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

Authors - Adrian Cuenca
humanWB

Cell Rep

RAB8B is required for activity and caveolar endocytosis of LRP6.

Authors - Kubilay Demir

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sara (Verified Customer) (04-07-2020)

I got a quite similar staining to the one shown in the example figure of this product data sheet. Not very sharp staining (a bit all over the cell), but working. Next I will try in WB.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Spleen / B cells