Validation Data Gallery
|Positive WB detected in||mouse brain tissue, human heart tissue, mouse lung tissue, human brain tissue, mouse heart tissue, rat brain tissue|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12321-1-AP targets RAB3IP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB3IP fusion protein Ag2974|
|Full Name||RAB3A interacting protein (rabin3)|
|Calculated molecular weight||476 aa, 53 kDa|
|Observed molecular weight||53/51/43 kDa|
|GenBank accession number||BC015548|
|Gene ID (NCBI)||117177|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles. This antibody can recognize isoforms with predicted MW's of 53 kDa, 51 kDa, 43 kDa, 45 kDa, 33 kDa and 35 kDa, 29 kDa respectively.
|Product Specific Protocols|
|WB protocol for RAB3IP antibody 12321-1-AP||Download protocol|
|IHC protocol for RAB3IP antibody 12321-1-AP||Download protocol|
|IF protocol for RAB3IP antibody 12321-1-AP||Download protocol|
|IP protocol for RAB3IP antibody 12321-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cytoplasmic control of Rab family small GTPases through BAG6.
Guanine nucleotide exchange factors activate Rab8a for Toll-like receptor signalling.
J Biol Chem
Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.
Rabin8 Protein Interacts with GTPase Rheb and Inhibits Phosphorylation of Ser235/Ser236 in Small Ribosomal Subunit Protein S6.
J Biol Chem
The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.
RAB8B is required for activity and caveolar endocytosis of LRP6.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Silvia (Verified Customer) (09-30-2021)
Immunofluorescence (1:200) works well on NPC
Sara (Verified Customer) (04-07-2020)
I got a quite similar staining to the one shown in the example figure of this product data sheet. Not very sharp staining (a bit all over the cell), but working. Next I will try in WB.