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  • KD/KO Validated

RAB3IP Polyclonal antibody

RAB3IP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12321-1-AP

Synonyms

Rab 3A interacting protein, Rab3A interacting protein, RAB3IP, RAB3IP/Rabin8, Rabin 3, RABIN3, Rabin8, SSX2 interacting protein



Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, mouse lung tissue, human brain tissue, mouse heart tissue, rat brain tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12321-1-AP targets RAB3IP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB3IP fusion protein Ag2974
Full Name RAB3A interacting protein (rabin3)
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 53/51/43 kDa
GenBank accession numberBC015548
Gene symbol RAB3IP
Gene ID (NCBI) 117177
RRIDAB_2177510
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles. This antibody can recognize isoforms with predicted MW's of 53 kDa, 51 kDa, 43 kDa, 45 kDa, 33 kDa and 35 kDa, 29 kDa respectively.

Protocols

Product Specific Protocols
WB protocol for RAB3IP antibody 12321-1-APDownload protocol
IHC protocol for RAB3IP antibody 12321-1-APDownload protocol
IF protocol for RAB3IP antibody 12321-1-APDownload protocol
IP protocol for RAB3IP antibody 12321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB,IF

Nat Cell Biol

A molecular network for de novo generation of the apical surface and lumen.

Authors - Bryant David M DM
  • KD Validated
ratWB, IF

Neuron

Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.

Authors - Sila K Ultanir
mouseWB, IF

PLoS Biol

GSK3β-Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis.

Authors - Boyan Zhang
humanWB

J Cell Biol

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

Authors - Michael C Burke
humanWB

EMBO J

Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.

Authors - Yu-Chiang Lai
humanWB

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

  • Applications: Immunofluorescence
FH

Sara (Verified Customer) (04-07-2020)

I got a quite similar staining to the one shown in the example figure of this product data sheet. Not very sharp staining (a bit all over the cell), but working. Next I will try in WB.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Spleen / B cells