RAB3IP Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB3IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (2)

Cat no : 12321-1-AP

Synonyms

RAB3IP/Rabin8, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein

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Validation Data Gallery

• 

  WB analysis using 12321-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12321-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12321-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12321-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12321-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12321-1-AP

  IP result of anti-RAB3IP (IP:12321-1-AP, 3ug; Detection:12321-1-AP 1:1000) with mouse lung tissue lysate 4500ug.

• 

  IHC staining of human stomach cancer using 12321-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12321-1-AP (RAB3IP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12321-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RAB3IP/Rabin8 antibody (12321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, mouse lung tissue, human brain tissue, mouse heart tissue, rat brain tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 16 publications below
• IF: See 6 publications below
• IP: See 3 publications below

Product Information

12321-1-AP targets RAB3IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB3IP fusion protein Ag2974
• Full Name: RAB3A interacting protein (rabin3)
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 53/51/43 kDa
• GenBank Accession Number: BC015548
• Gene Symbol: RAB3IP
• Gene ID (NCBI): 117177
• RRID: AB_2177510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles. This antibody can recognize isoforms with predicted MW's of 53 kDa, 51 kDa, 43 kDa, 45 kDa, 33 kDa and 35 kDa, 29 kDa respectively.

Publications

Species	Application	Title
canine	WB,IF	Nat Cell Biol; A molecular network for de novo generation of the apical surface and lumen.; Authors - Bryant David M DM ; KD Validated
rat	WB, IF	Neuron; Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.; Authors - Sila K Ultanir
mouse	WB, IF	PLoS Biol; GSK3β-Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis.; Authors - Boyan Zhang
human	WB	J Cell Biol; Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.; Authors - Michael C Burke
human	WB	EMBO J; Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.; Authors - Yu-Chiang Lai
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

• Applications: Immunofluorescence

FH

Sara (Verified Customer) (04-07-2020)

I got a quite similar staining to the one shown in the example figure of this product data sheet. Not very sharp staining (a bit all over the cell), but working. Next I will try in WB.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Spleen / B cells