Validation Data Gallery
|Positive WB detected in
|mouse brain tissue, human heart tissue, mouse lung tissue, human brain tissue, mouse heart tissue, rat brain tissue
|Positive IP detected in
|mouse lung tissue
|Positive IHC detected in
|human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:3000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12321-1-AP targets RAB3IP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, canine
|Host / Isotype
|Rabbit / IgG
|RAB3IP fusion protein Ag2974
|RAB3A interacting protein (rabin3)
|Calculated molecular weight
|476 aa, 53 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles. This antibody can recognize isoforms with predicted MW's of 53 kDa, 51 kDa, 43 kDa, 45 kDa, 33 kDa and 35 kDa, 29 kDa respectively.
|Product Specific Protocols
|WB protocol for RAB3IP antibody 12321-1-AP
|IHC protocol for RAB3IP antibody 12321-1-AP
|IF protocol for RAB3IP antibody 12321-1-AP
|IP protocol for RAB3IP antibody 12321-1-AP
|Click here to view our Standard Protocols
Nat Cell Biol
A molecular network for de novo generation of the apical surface and lumen.
Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.
GSK3β-Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis.
J Cell Biol
Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.
Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.
Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Silvia (Verified Customer) (09-30-2021)
Immunofluorescence (1:200) works well on NPC
Sara (Verified Customer) (04-07-2020)
I got a quite similar staining to the one shown in the example figure of this product data sheet. Not very sharp staining (a bit all over the cell), but working. Next I will try in WB.