CoraLite®594-conjugated RAB3IP/Rabin8 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB3IP/Rabin8 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-12321

Synonyms

FLJ14660, FLJ22548, Rab 3A interacting protein, Rab3A interacting protein, RAB3IP, Rabin 3, RABIN3, rabin8, SSX2 interacting protein

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-12321

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 RAB3IP/Rabin8 antibody (CL594-12321) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-12321 targets RAB3IP/Rabin8 in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB3IP/Rabin8 fusion protein Ag2974
• Full Name: RAB3A interacting protein (rabin3)
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC015548
• Gene Symbol: RAB3IP
• Gene ID (NCBI): 117177
• RRID: AB_2919782
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles.