RAB7A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB7A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

61

Reviews (6)

Cat no : 55469-1-AP

Synonyms

RAB7, Ras-related protein Rab-7a, Ras related protein Rab 7a, EC:3.6.5.2

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Validation Data Gallery

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  WB analysis using 55469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 55469-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55469-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55469-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55469-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB7A antibody (55469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 55469-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RAB7A (55469-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: L-929 cells, NIH/3T3 cells, A431 cells, SH-SY5Y cells, RAW 264.7 cells, C6 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human kidney tissue, human skeletal muscle tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 44 publications below
• IHC: See 3 publications below
• IP: See 2 publications below

Product Information

55469-1-AP targets RAB7A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAB7A, member RAS oncogene family
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: NM_004637
• Gene Symbol: RAB7A
• Gene ID (NCBI): 7879
• RRID: AB_11182831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

Publications

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
Zebrafish,monkey		Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
		Nat Commun; CHML promotes liver cancer metastasis by facilitating Rab14 recycle.; Authors - Tian-Wei Chen
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sophy (Verified Customer) (03-07-2023)

The antibody worked well in western with 1:1000 dilution. The signal was somewhat weak but that could also be due to the cell types that were used.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSCLC cells

FH

Malgorzata (Verified Customer) (08-17-2022)

The antibody worked on my positive control: Lysate of THP1 cell line. Nevertheless, for my samples it gave strong nonspecific signal in high MW

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: THP-1

RAB7A Antibody Western Blot validation (1000 dilution) in THP-1 (Cat no:55469-1-AP)

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NSC-34 cells

FH

Laura (Verified Customer) (01-15-2020)

Works well for Western Blot at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Aamir (Verified Customer) (01-08-2020)

WB at 1 in 500IF at 1 in 250

• Applications: Western Blot, Immunohistochemistry,
• Cell Tissue Type: HEKs

FH

Benjamin (Verified Customer) (12-10-2019)

Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293 Lysate

RAB7A Antibody Western Blot, validation (1:1000 dilution) in HEK293 Lysate (Cat no:55469-1-AP)

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