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  • KD/KO Validated

RAB7A Polyclonal antibody

RAB7A Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55469-1-AP

Synonyms

PRO2706, RAB7, RAB7A, Ras related protein Rab 7a



Tested Applications

Positive WB detected inL-929 cells, SH-SY5Y cells, A431 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, C6 cells, RAW 264.7 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55469-1-AP targets RAB7A in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, pig, rat, Zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB7A, member RAS oncogene family
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberNM_004637
Gene symbol RAB7A
Gene ID (NCBI) 7879
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

Protocols

Product Specific Protocols
WB protocol for RAB7A antibody 55469-1-APDownload protocol
IHC protocol for RAB7A antibody 55469-1-APDownload protocol
IP protocol for RAB7A antibody 55469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Aging (Albany NY)

Cytoplasmic PCNA is located in the actin belt and involved in osteoclast differentiation.

Authors - Donge Tang
humanIF

Cell Death Dis

FV-429 induces autophagy blockage and lysosome-dependent cell death of T-cell malignancies via lysosomal dysregulation.

Authors - Po Hu
humanIF,WB

Cell Death Dis

TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.

Authors - Jia Hu
  • KD Validated
humanWB, IF

Signal Transduct Target Ther

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Authors - Wenchang Xiao
humanWB,IF

Mol Med Rep

Synergism between upregulation of Rab7 and inhibition of autophagic degradation caused by mycoplasma facilitates intracellular mycoplasma infection.

Authors - Xiaopeng Hu
ratWB

Mol Med Rep

Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy.

Authors - Zhongzhong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: NSC-34 cells
FH

Laura (Verified Customer) (01-15-2020)

Works well for Western Blot at 1:1000 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Aamir (Verified Customer) (01-08-2020)

WB at 1 in 500IF at 1 in 250

  • Applications: Western Blot,Immunohistochemistry,
  • Cell Tissue Type: HEKs
FH

Benjamin (Verified Customer) (12-10-2019)

Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293 Lysate
RAB7A Antibody Western Blot, validation (1:1000 dilution) in HEK293 Lysate (Cat no:55469-1-AP)