Validation Data Gallery
|Positive WB detected in||L-929 cells, NIH/3T3 cells, A431 cells, SH-SY5Y cells, RAW 264.7 cells, C6 cells, mouse brain tissue, rat brain tissue|
|Positive IHC detected in||human kidney tissue, human skeletal muscle tissue, human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||A431 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55469-1-AP targets RAB7A in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey, Zebrafish, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||RAB7A, member RAS oncogene family|
|Calculated molecular weight||23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||NM_004637|
|Gene ID (NCBI)||7879|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.
RAB31 marks and controls an ESCRT-independent exosome pathway.
C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy
SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes
Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.
CHML promotes liver cancer metastasis by facilitating Rab14 recycle.
Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sophy (Verified Customer) (03-07-2023)
The antibody worked well in western with 1:1000 dilution. The signal was somewhat weak but that could also be due to the cell types that were used.
Malgorzata (Verified Customer) (08-17-2022)
The antibody worked on my positive control: Lysate of THP1 cell line. Nevertheless, for my samples it gave strong nonspecific signal in high MW
Azita (Verified Customer) (06-17-2021)
Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.
Laura (Verified Customer) (01-15-2020)
Works well for Western Blot at 1:1000 dilution.
Aamir (Verified Customer) (01-08-2020)
WB at 1 in 500IF at 1 in 250
Benjamin (Verified Customer) (12-10-2019)
Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.