RABEPK/p40 Monoclonal antibody

RABEPK/p40 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1E11A3

Cat no : 66622-1-Ig

Synonyms

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells, HSCT6 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66622-1-Ig targets RABEPK/p40 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RABEPK/p40 fusion protein Ag7796
Full Name Rab9 effector protein with kelch motifs
Calculated molecular weight 41 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC065725
Gene symbol RABEPK
Gene ID (NCBI) 10244
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Protocols

Product Specific Protocols
WB protocol for RABEPK/p40 antibody 66622-1-IgDownload protocol
IHC protocol for RABEPK/p40 antibody 66622-1-IgDownload protocol
IF protocol for RABEPK/p40 antibody 66622-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols