RAGE Polyclonal antibody

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  WB analysis using 31122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31122-1-AP (RAGE antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 31122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31122-1-AP (RAGE antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAGE Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31122-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Ager, Advanced glycosylation end product-specific receptor, MOK, RAGE 1, RAGE1

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31122-1-AP targets RAGE in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAGE fusion protein Eg0873
• Full Name: advanced glycosylation end product-specific receptor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42-55 kDa
• GenBank Accession Number: NM_007425.3
• Gene Symbol: Ager
• Gene ID (NCBI): 11596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q62151-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).