|Positive WB detected in||COLO 320 cells, HeLa cells, U2OS cells, HUVEC cells, NCCIT cells, human spleen tissue, DC2.4 cells, JAR cells, RAW 264.7 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Sample-dependent, check data in validation data gallery|
66610-1-Ig targets RANKL in WB, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||RANKL fusion protein Ag19975|
|Full Name||tumor necrosis factor (ligand) superfamily, member 11|
|Calculated molecular weight||317 aa, 35 kDa|
|Observed molecular weight||35-38 kDa|
|GenBank accession number||BC074890|
|Gene ID (NCBI)||8600|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.