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RANKL Monoclonal antibody

RANKL Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat and More (1)







Cat no : 66610-1-Ig


CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoprotegerin ligand, RANKL, sOdf, TNFSF11, TRANCE

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, U2OS cells, HUVEC cells, NCCIT cells, human spleen tissue, DC2.4 cells, JAR cells, RAW 264.7 cells
Positive IF detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66610-1-Ig targets RANKL in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RANKL fusion protein Ag19975
Full Name tumor necrosis factor (ligand) superfamily, member 11
Calculated Molecular Weight 317 aa, 35 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC074890
Gene Symbol TNFSF11
Gene ID (NCBI) 8600
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.


Product Specific Protocols
WB protocol for RANKL antibody 66610-1-IgDownload protocol
IF protocol for RANKL antibody 66610-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Cancer

Gut microbiota deficiency ameliorates multiple myeloma and myeloma-related bone disease by Th17 cells in mice models

Authors - Jia Liu
humanWB, IF

Cell Death Dis

RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis.

Authors - Qian Liang

Open Life Sci

Effect of periostin on bone metabolic and autophagy factors during tooth eruption in mice

Authors - Han Qin

J Periodontal Res

Role of Piezo1 in modulating the RANKL/OPG ratio in mouse osteoblast cells exposed to Porphyromonas gingivalis lipopolysaccharide and mechanical stress

Authors - Mabuki Uchinuma