RBBP9 Polyclonal antibody

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  WB analysis of mouse testis using 12230-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12230-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 12230-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12230-2-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12230-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 12230-2-AP

  IP result of anti-RBBP9 (IP:12230-2-AP, 3ug; Detection:12230-2-AP 1:300) with mouse lung tissue lysate 5160ug.

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  IHC staining of human pancreas cancer using 12230-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12230-2-AP (RBBP9 antibody) at dilution of 1:50 (under 10x lens).

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  FC experiment of BxPC-3 using 12230-2-AP

  1x10^6 BxPC-3 cells were intracellularly stained with 0.4 ug RBBP9 Polyclonal antibody (12230-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

RBBP9 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 12230-2-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

B5T-overexpressed gene protein, BOG, EC:3.-.-.-, Glutathione S-Transferase, RBBP 10

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Tested Applications

• Positive WB detected in: mouse testis tissue, A375 cells, human lung tissue, human spleen tissue, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

12230-2-AP targets RBBP9 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2868

  Product name: Recombinant human RBBP9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-186 aa of BC015938

  Sequence: MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHRVYAIVLVSAYTSDLGDENERASGYFTRPWQWEKIKANCPYIVQFGSTDDPFLPWKEQQEVADRLETKLHKFTDCGHFQNTEFHELITVVKSLLKVPA

• Full Name: retinoblastoma binding protein 9
• Calculated Molecular Weight: 186 aa, 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC015938
• Gene Symbol: RBBP9
• Gene ID (NCBI): 10741
• RRID: AB_2175265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75884
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID: 20080647) The genes CyFIP2 and RbBP9, which are also miss-expressed in ALS hMSC, could serve as diagnostic biomarker tools for detection of ALS in blood samples.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Proc Natl Acad Sci U S A; RBBP9: a tumor-associated serine hydrolase activity required for pancreatic neoplasia.; Authors - Shields David J DJ
mouse	WB,IHC	Cell Mol Gastroenterol Hepatol; Retinoblastoma-Binding Protein 9 Suppresses Intestinal Inflammation and Inflammation-Induced Tumorigenesis in Mice; Authors - Kensuke Hamada ; KD Validated
mouse,human	WB	Antioxid Redox Signal; Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins.; Authors - Maya A Olshina
human	WB	Exp Hematol; Retinoblastoma-binding proteins 4 and 9 are important for human pluripotent stem cell maintenance.; Authors - Michael D O'Connor ; KD Validated
human	WB	Dis Markers; Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis.; Authors - Nachmany Henny H