Tested Applications
| Positive WB detected in | HEK-293 cells, HEK293 cells, HT-29 cells, HeLa cells, SW 480 cells, SW480 cells, BV-2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10587-1-AP targets RBM15 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0938 Product name: Recombinant human RBM15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 658-957 aa of BC006397 Sequence: SSSRDRYNSDNDRSSRLLLERPSPIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQDGGTAPVASASPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQPKLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSAASDTATSTQRPLRNLVSYLKQKQAAGVISLPVGGNKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRGFGFQIGVRYENKKRENLALTLL Predict reactive species |
| Full Name | RNA binding motif protein 15 |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 100-107 kDa |
| GenBank Accession Number | BC006397 |
| Gene Symbol | RBM15 |
| Gene ID (NCBI) | 64783 |
| RRID | AB_2175757 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96T37 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBM15 antibody 10587-1-AP | Download protocol |
| IHC protocol for RBM15 antibody 10587-1-AP | Download protocol |
| IP protocol for RBM15 antibody 10587-1-AP | Download protocol |
| WB protocol for RBM15 antibody 10587-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RBM15 Polyclonal antibody (Cat# 10587-1-AP) has 81 citations. These appear in journals including Nature, Cell, Nature Cell Biology, Nature Neuroscience, Nature Communications, Molecular Cell, Developmental Cell, Cancer Research, JBC, PLoS One, eLife, and Frontiers in Immunology, among others. Research fields span m6A RNA modification, cancer biology (gastric, lung, ovarian, prostate, and other cancers), X-chromosome inactivation, RNA splicing and export, virology (KSHV, HIV, HBV), neurology, and metabolism. Applications include WB, IHC, IF, IP, CoIP, and RIP.
| Species | Application | Title |
|---|---|---|
Cell MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids | ||
Mol Cancer Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer | ||
Nat Cell Biol RBFOX2 recognizes N6-methyladenosine to suppress transcription and block myeloid leukaemia differentiation | ||
Cancer Res Neuronal Activity Promotes Glioma Progression by Inducing Proneural-to-Mesenchymal Transition in Glioma Stem Cells | ||
Drug Resist Updat RNA-binding motif protein 15 promotes gastric cancer growth and drug resistance via USP10-mediated deubiquitination and stabilization of nuclear NRF2.
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Reviews
What customers say
The product received one review with a 5-star rating. The reviewer used it for Western Blot at a 1:1000 dilution on cancer cells and reported that it "works great," indicating strong performance and satisfaction with the antibody's performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sonam (Verified Customer) (01-27-2026) | Works great
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