RBM15 Polyclonal antibody

• Featured Product
• KD/KO Validated

RBM15 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

37

Cat no : 10587-1-AP

Synonyms

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

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Validation Data Gallery

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  WB analysis of HEK293 cells using 10587-1-AP

  WB result of RBM15 antibody (10587-1-AP, 1:3000) with si-control and si-RBM15 transfected HEK293 cells.

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  WB analysis of HEK-293 using 10587-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 10587-1-AP

  SW480 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW480 using 10587-1-AP

  SW480 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10587-1-AP

  IP result of anti-RBM15 (IP:10587-1-AP, 4ug; Detection:10587-1-AP 1:500) with HEK-293 cells lysate 2600 ug.

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  IHC staining of human breast cancer using 10587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10587-1-AP (RBM15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10587-1-AP (RBM15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 10587-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM15 antibody (10587-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: SW480 cells, HEK293 cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 29 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Product Information

10587-1-AP targets RBM15 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM15 fusion protein Ag0938
• Full Name: RNA binding motif protein 15
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 100-107 kDa
• GenBank Accession Number: BC006397
• Gene Symbol: RBM15
• Gene ID (NCBI): 64783
• RRID: AB_2175757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Publications

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu ; KO Validated
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB,IF,RIP,CoIP	Dev Cell; N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.; Authors - Xinyu Wang ; KD Validated
human	WB	Elife; Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.; Authors - Li Zhang
human	WB,IF,IP	Nucleic Acids Res; Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA.; Authors - Zolotukhin Andrei S AS
mouse	IF	Cell Rep; A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.; Authors - Benoit Moindrot