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  • KD/KO Validated

RBM15 Polyclonal antibody

RBM15 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 10587-1-AP

Synonyms

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN



Tested Applications

Positive WB detected inHEK-293 cells, HEK293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10587-1-AP targets RBM15 in WB, RIP, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM15 fusion protein Ag0938
Full Name RNA binding motif protein 15
Calculated molecular weight 105 kDa
Observed molecular weight 100-107 kDa
GenBank accession numberBC006397
Gene symbol RBM15
Gene ID (NCBI) 64783
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Protocols

Product Specific Protocols
WB protocol for RBM15 antibody 10587-1-APDownload protocol
IHC protocol for RBM15 antibody 10587-1-APDownload protocol
IF protocol for RBM15 antibody 10587-1-APDownload protocol
IP protocol for RBM15 antibody 10587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Mol Neurobiol

RBM15 Modulates the Function of Chromatin Remodeling Factor BAF155 Through RNA Methylation in Developing Cortex.

Authors - Yuanbin Xie
  • KD Validated
humanIHC

Med Sci Monit

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.

Authors - Yingying Zhang
humanWB

Wellcome Open Res

The role of the Xist 5' m6A region and RBM15 in X chromosome inactivation.

Authors - Heather Coker
humanIHC

J Exp Clin Cancer Res

RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent.

Authors - Xin Wang
humanWB

PLoS One

Splicing factor SF3B1K700E mutant dysregulates erythroid differentiation via aberrant alternative splicing of transcription factor TAL1.

Authors - Shuiling Jin
  • KD Validated
humanWB

Virology

Requirement of UAP56, URH49, RBM15, and OTT3 in the expression of Kaposi sarcoma-associated herpesvirus ORF57.

Authors - Majerciak Vladimir V