|Positive WB detected in||HEK-293 cells, HEK293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10587-1-AP targets RBM15 in WB, RIP, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RBM15 fusion protein Ag0938|
|Full Name||RNA binding motif protein 15|
|Calculated molecular weight||105 kDa|
|Observed molecular weight||100-107 kDa|
|GenBank accession number||BC006397|
|Gene ID (NCBI)||64783|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.
|Product Specific Protocols|
|WB protocol for RBM15 antibody 10587-1-AP||Download protocol|
|IHC protocol for RBM15 antibody 10587-1-AP||Download protocol|
|IF protocol for RBM15 antibody 10587-1-AP||Download protocol|
|IP protocol for RBM15 antibody 10587-1-AP||Download protocol|
|Click here to view our Standard Protocols|
RBM15 Modulates the Function of Chromatin Remodeling Factor BAF155 Through RNA Methylation in Developing Cortex.
Med Sci Monit
Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.
Wellcome Open Res
The role of the Xist 5' m6A region and RBM15 in X chromosome inactivation.
J Exp Clin Cancer Res
RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent.
Splicing factor SF3B1K700E mutant dysregulates erythroid differentiation via aberrant alternative splicing of transcription factor TAL1.
Requirement of UAP56, URH49, RBM15, and OTT3 in the expression of Kaposi sarcoma-associated herpesvirus ORF57.