CoraLite®594-conjugated RBM15 Polyclonal antibody

• Featured Product
• KD/KO Validated

RBM15 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10587

Synonyms

FLJ12479, FLJ21943, One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-10587

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RBM15 antibody (CL594-10587) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10587 targets RBM15 in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM15 fusion protein Ag0938
• Full Name: RNA binding motif protein 15
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 100-107 kDa
• GenBank Accession Number: BC006397
• Gene Symbol: RBM15
• Gene ID (NCBI): 64783
• RRID: AB_2919738
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.