RBP2 Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18825-1-AP (RBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 18825-1-AP (same clone as 18825-1-PBS)

  WB result of KDM5A antibody (18825-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and KDM5A knockout HEK-293T cells. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

  IP result of anti-RBP2 (IP:18825-1-AP, 4ug; Detection:18825-1-AP 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18825-1-AP (RBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RBP2 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, Indirect ELISA

Cat No. 18825-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

KDM5A, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, EC:1.14.11.67, JARID1A, Jumonji/ARID domain-containing protein 1A

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Product Information

18825-1-PBS targets RBP2 in WB, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: lysine (K)-specific demethylase 5A
• Calculated Molecular Weight: 192 kDa
• Observed Molecular Weight: 192 kDa
• GenBank Accession Number: NM_005056
• Gene Symbol: KDM5A
• Gene ID (NCBI): 5927
• RRID: AB_10638436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29375
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.