Mouse RBP4 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01365-1

  Sandwich ELISA standard curve of MP01365-1, Mouse RBP4 Recombinant Matched Antibody Pair - PBS only. 84503-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1140. 84503-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP01365-1

  Urine of six mice was measured. The mouse RBP4 concentration of detected samples was determined to be 7.25 ng/mL with a range of 2.50 - 12.78 ng/mL.

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  Sample test of MP01365-1

  Mouse kidney tissue from one adult mouse (chopped in 1-2 mm piecesin 30 mL of medium) was cultured in RPMI supplemented with 10% fetal calfserum and 50 μM β-mercaptoethanol. Aliquots of the cell culture supernates were removed, assayed for levels of mouse RBP4, and measured 14.68 ng/mL.

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  Sample test of MP01365-1

  The mean RBP4 concentration was determined to be 45.00 ng/mL in mouse kidney tissue extract based on a 1.9 mg/mL extract load.

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  Sample test of MP01365-1

  Serum of six mice was measured. The mouse RBP4 concentration of detected samples was determined to be 4,970.53 ng/mL with a range of 2,551.06 - 5,913.45 ng/mL.

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  Cytometric bead array standard curve of MP01365-1

  Cytometric bead array standard curve of MP01365-1, MOUSE RBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84503-1-PBS. Detection antibody: 84503-3-PBS. Standard: Eg1140. Range: 0.156-10 ng/mL.

RBP4 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84503-3-PBS

Clone No.242040G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Plasma retinol-binding protein, PRBP, RBP, Retinol-binding protein 4

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Product Information

84503-3-PBS targets RBP4 as part of a matched antibody pair:

MP01365-1: 84503-1-PBS capture and 84503-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RBP4 fusion protein Eg1140
• Full Name: retinol binding protein 4, plasma
• Calculated Molecular Weight: 23kDa
• GenBank Accession Number: NM_011255.3
• Gene Symbol: Rbp4
• Gene ID (NCBI): 19662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00724
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.