RBPJ Recombinant antibody

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  WB analysis using 85676-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85676-3-RR (RBPJ antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

RBPJ Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85676-3-RR

Clone No.243044G4

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

CBF 1, CBF1, CBF-1, csl, IGKJRB

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cell, HEK-293 cells, Jurkat cells, MCF-7 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85676-3-RR targets RBPJ in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RBPJ fusion protein Ag32552
• Full Name: recombination signal binding protein for immunoglobulin kappa J region
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: ~60 kDa
• GenBank Accession Number: BC064976
• Gene Symbol: RBPJ
• Gene ID (NCBI): 3516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q06330
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.