RIOK3 Polyclonal antibody

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  WB analysis using 13593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13593-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13593-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13593-1-AP

  IP result of anti-RIOK3 (IP:13593-1-AP, 4ug; Detection:13593-1-AP 1:600) with mouse testis tissue lysate 4000ug.

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  IHC staining of human breast cancer using 13593-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13593-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13593-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A431 using 13593-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

RIOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13593-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

sudD homolog, SUDD, Serine/threonine-protein kinase RIO3, RIO kinase 3 (yeast), RIO kinase 3

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse testis tissue, MCF-7 cells, rat testis tissue, HeLa cells, Jurkat cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below

Product Information

13593-1-AP targets RIOK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4497

  Product name: Recombinant human RIOK3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 215-516 aa of BC039729

  Sequence: RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE

• Full Name: RIO kinase 3 (yeast)
• Calculated Molecular Weight: 516 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC039729
• Gene Symbol: RIOK3
• Gene ID (NCBI): 8780
• RRID: AB_2178105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14730
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIOK3(Serine/threonine-protein kinase RIO3) is initially characterized as a homolog of Aspergillus nidulans sudD and showed down-regulation at the invasive front of malignant melanomas.On Western blots, human RIOK3 migrated as a 60-kD protein. Northern blot analysis indicated that the approximately 4-kb RIOK3 mRNA is expressed widely in human tissues, with additional smaller transcripts present in testis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.; Authors - Nandan S Gokhale
mouse	WB	Genes Dev; miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1.; Authors - Lingbo Zhang
human	WB	Biomedicines; RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis.; Authors - Min Yu
mouse	WB	Cell Rep; Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.; Authors - Yong Shen ; KO Validated
human	WB	Mol Cell; The integrated stress response regulates 18S nonfunctional rRNA decay in mammals; Authors - Aaztli R Coria
human	WB	Mol Cell; RIOK3 mediates the degradation of 40S ribosomes; Authors - Zixuan Huang ; KO Validated