|Positive WB detected in||Jurkat cells, NIH/3T3 cells|
|Positive IP detected in||SW 1990 cells|
|Positive IHC detected in||human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
17563-1-AP targets RIP3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RIP3 fusion protein Ag11759|
|Full Name||receptor-interacting serine-threonine kinase 3|
|Calculated molecular weight||518 aa, 57 kDa|
|Observed molecular weight||57 kDa|
|GenBank accession number||BC062584|
|Gene ID (NCBI)||11035|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Receptor-interacting protein 3 (RIP3, also known as RIPK3) is a serine-threonine protein involved in the regulation
of inflammatory signaling and cell death. RIPK3, also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting
Protein) family, is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal
(NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus (PMID:18533105). It has 3 isoforms produced by
What is the molecular weight of RIP3? Is RIP3 post-translationally modified?
The molecular weight of RIP3 is 57 kDa. During the induction of necroptosis, RIP3 migrates slower in SDS-PAGE
due to its phosphorylation (PMID: 19524512). Additionally, RIP3 can be a subject of poly-ubiquitination, when
targeted for degradation.
Are there any splice isoforms of RIP3?
Apart from full-length RIP3, there are two reported splice isoforms of RIP3: RIP3β and RIP3γ, and 28 and 25 kDa,
respectively (PMID: 15896315).
What is the subcellular localization of RIP3?
RIP3 can shuttle between the cytoplasm and the nucleus. Although RIP3 forms necrosomes in the cytoplasm, a
recent study suggests that the phosphorylation of RIP3, required for necroptosis, may also occur in the nucleus
(PMID: 30271893). Additionally, the induction of necrosis by reactive oxygen species can cause transient
translocation of RIP3 to mitochondria (PMID: 25206339).
What is the role of RIP3 in cell death (necroptosis)?
Activation of RIP3 kinase is required for the induction of necroptosis (PMID: 19524512, 19524513 and 19498109).
Activation of RIP3 can be induced by interferons, death ligands, or by Toll-like receptors in response to pathogens.
That leads to the phosphorylation of RIP3 and the formation of a β-amyloid-like protein complex. Phosphorylated
RIP3 acts downstream by phosphorylation of MLKL (PMID: 30131615).
How to study necroptosis in a cell-based system
The choice of the cell line is important. Many commonly used immortalized cell types are derived from cancers
and may have very low RIL3 expression level (PMID: 25952668). Those cell lines are not going to be responsive to
necrotic stimuli. A few of the examined cell types have high RIP3 levels: Jurkat, CCRF-CEM, U937, L929 cells, and
mouse embryonic fibroblasts (PMID: 19524512). Good necroptosis readouts reflect an increased level of RIP3
protein and its phosphorylation.
J Cell Physiol
MUC1 downregulation promotes TNF-α-induced necroptosis in human bronchial epithelial cells via regulation of the RIPK1/RIPK3 pathway.
Cardiovasc Drugs Ther
Cell Death Differ
The role of the LncRNA-FA2H-2-MLKL pathway in atherosclerosis by regulation of autophagy flux and inflammation through mTOR-dependent signaling.
Hum Gene Ther
A "Hibernating-like" viable state induced by Lentiviral Vector-Mediated PEDF overexpression in rat acute ischemic myocardium.
Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis.
TNF-α increases Staphylococcus aureus-induced death of human alveolar epithelial cell line A549 associated with RIP3-mediated necroptosis.