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RIP3 Polyclonal antibody

RIP3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17563-1-AP

Synonyms

Receptor interacting protein 3, RIP 3, RIP like protein kinase 3, RIP3, RIPK3



Tested Applications

Positive WB detected inHT-29 cells, Jurkat cells, HeLa cells, SW 1990 cells, THP-1 cells, SGC-7901 cells
Positive IP detected inSW 1990 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

17563-1-AP targets RIP3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIP3 fusion protein Ag11759
Full Name receptor-interacting serine-threonine kinase 3
Calculated molecular weight 518 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC062584
Gene symbol RIPK3
Gene ID (NCBI) 11035
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information


Receptor-interacting protein 3 (RIP3, also known as RIPK3) is a serine-threonine protein involved in the regulation 

of inflammatory signaling and cell death. RIPK3, also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting 

Protein) family, is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal 

(NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus (PMID:18533105). It has 3 isoforms produced by 

alternative splicing.


What is the molecular weight of RIP3? Is RIP3 post-translationally modified?

The molecular weight of RIP3 is 57 kDa. During the induction of necroptosis, RIP3 migrates slower in SDS-PAGE 

due to its phosphorylation (PMID: 19524512). Additionally, RIP3 can be a subject of poly-ubiquitination, when 

targeted for degradation.


Are there any splice isoforms of RIP3?

Apart from full-length RIP3, there are two reported splice isoforms of RIP3: RIP3β and RIP3γ, and 28 and 25 kDa, 

respectively (PMID: 15896315).


What is the subcellular localization of RIP3?

RIP3 can shuttle between the cytoplasm and the nucleus. Although RIP3 forms necrosomes in the cytoplasm, a 

recent study suggests that the phosphorylation of RIP3, required for necroptosis, may also occur in the nucleus 

(PMID: 30271893). Additionally, the induction of necrosis by reactive oxygen species can cause transient 

translocation of RIP3 to mitochondria (PMID: 25206339).


What is the role of RIP3 in cell death (necroptosis)?

Activation of RIP3 kinase is required for the induction of necroptosis (PMID: 19524512, 19524513 and 19498109). 

Activation of RIP3 can be induced by interferons, death ligands, or by Toll-like receptors in response to pathogens. 

That leads to the phosphorylation of RIP3 and the formation of a β-amyloid-like protein complex. Phosphorylated 

RIP3 acts downstream by phosphorylation of MLKL (PMID: 30131615).


How to study necroptosis in a cell-based system

The choice of the cell line is important. Many commonly used immortalized cell types are derived from cancers 

and may have very low RIL3 expression level (PMID: 25952668). Those cell lines are not going to be responsive to 

necrotic stimuli. A few of the examined cell types have high RIP3 levels: Jurkat, CCRF-CEM, U937, L929 cells, and 

mouse embryonic fibroblasts (PMID: 19524512). Good necroptosis readouts reflect an increased level of RIP3 

protein and its phosphorylation.


Protocols

Product Specific Protocols
WB protocol for RIP3 antibody 17563-1-APDownload protocol
IHC protocol for RIP3 antibody 17563-1-APDownload protocol
IP protocol for RIP3 antibody 17563-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Environ Pollut

Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium.

Authors - Jianqiang Zhu
mouse

Cell Mol Life Sci

Thioredoxin reductase 3 suppression promotes colitis and carcinogenesis via activating pyroptosis and necrosis.

Authors - Qi Liu
mouseIF

J Neurosci

PMID: 30733218

Authors - Douglas Ruhl
pigWB

Front Cell Dev Biol

Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model.

Authors - Jianni Huang
mouseWB

FASEB J

LncRNA 122049 suppresses apoptosis of renal tubular epithelial cells in ischemic AKI by targeting the miR-330-5p/ELK1 axis.

Authors - Ying Xiao
ratWB

FASEB J

Protective effect of inhibiting necroptosis on gentamicin-induced nephrotoxicity

Authors - Bing-Feng Hu