RPA2 Monoclonal antibody

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  WB analysis using 67999-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of pig spleen using 67999-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 67999-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 67999-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit spleen using 67999-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 67999-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

RPA2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67999-1-Ig

Clone No.1B9H11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2

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Tested Applications

• Positive WB detected in: LNCaP cells, pig spleen tissue, rat testis tissue, mouse testis tissue, rabbit spleen tissue, Hela cells, MOLT-4 cells, Jurkat cells, THP-1 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67999-1-Ig targets RPA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24783

  Product name: Recombinant human RPA2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 80-270 aa of BC001630

  Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

• Full Name: replication protein A2, 32kDa
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC001630
• Gene Symbol: RPA2
• Gene ID (NCBI): 6118
• RRID: AB_2918747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P15927
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.