RPL10A Recombinant antibody

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  WB analysis using 85685-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85685-1-RR (RPL10A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85685-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL10A antibody (85685-1-RR, Clone: 243031C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RPL10A Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85685-1-RR

Clone No.243031C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

60S ribosomal protein L10a, Csa 19, CSA-19, Large ribosomal subunit protein uL1, NEDD 6

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, HepG2 cells, HeLa cells, mouse spleen tissue, rat spleen tissue, fetal human brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85685-1-RR targets RPL10A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPL10A fusion protein Ag10009
• Full Name: ribosomal protein L10a
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC006791
• Gene Symbol: RPL10A
• Gene ID (NCBI): 4736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62906
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL10A (Ribosomal Protein L10a) is a component of the 60S subunit of the ribosome and belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. RPL10A is ubiquitously expressed in various tissues, it plays a crucial role in ribosome biogenesis and protein synthesis. In addition to its role in protein synthesis, RPL10A has been implicated in cell proliferation, embryogenesis, and organogenesis. PMID: 29953919