RPL27 Polyclonal antibody

RPL27 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14980-1-AP

Synonyms

60S ribosomal protein L27, ribosomal protein L27, RPL27



Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14980-1-AP targets RPL27 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL27 fusion protein Ag6893
Full Name ribosomal protein L27
Calculated molecular weight 16 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC001700
Gene symbol RPL27
Gene ID (NCBI) 6155
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPL27, also named as 60S ribosomal protein L27, is a 136 amino acid protein, which contains one KOW domain and belongs to the ribosomal protein L27e family. The function of RPL27 is still non-known. RPL27 may play a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome.

Protocols

Product Specific Protocols
WB protocol for RPL27 antibody 14980-1-APDownload protocol
IHC protocol for RPL27 antibody 14980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Rep

miR-132 suppresses transcription of ribosomal proteins to promote protective Th1 immunity.

Authors - James P Hewitson
ratWB

Physiol Rep

Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules.

Authors - Muhammad U Cheema
humanIHC

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis