|Positive WB detected in||mouse liver tissue, human liver tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14980-1-AP targets RPL27 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RPL27 fusion protein Ag6893|
|Full Name||ribosomal protein L27|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC001700|
|Gene ID (NCBI)||6155|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RPL27, also named as 60S ribosomal protein L27, is a 136 amino acid protein, which contains one KOW domain and belongs to the ribosomal protein L27e family. The function of RPL27 is still non-known. RPL27 may play a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome.
miR-132 suppresses transcription of ribosomal proteins to promote protective Th1 immunity.
Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules.
Proc Natl Acad Sci U S A
Local translation in nuclear condensate amyloid bodies.