RPL5 Polyclonal antibody

RPL5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Monkey

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 29092-1-AP

Synonyms

60S ribosomal protein L5, DBA6, MSTP030, ribosomal protein L5, RPL5



Tested Applications

Positive WB detected inCOS-7 cells, MOLT-4 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

29092-1-AP targets RPL5 in WB, IHC,ELISA applications and shows reactivity with Human, Monkey samples.

Tested Reactivity Human, Monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL5 fusion protein Ag30837
Full Name ribosomal protein L5
Observed molecular weight34 kDa
GenBank accession numberBC109370
Gene symbol RPL5
Gene ID (NCBI) 6125
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian ribosome is a macromolecular assembly of 4 RNA species (rRNAs) and approximately 80 different proteins, including RPL5. RPL5 (ribosome protein L15) is rquired for rRNA maturation and formation of the 60S ribosomal subunits, and defects or mutation in RPL5 is the cause of DBA6.

Protocols

Product Specific Protocols
WB protocol for RPL5 antibody 29092-1-APDownload protocol
IHC protocol for RPL5 antibody 29092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols