RSL24D1 Polyclonal antibody

• 

  WB analysis of HepG2 using 25190-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25190-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 25190-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25190-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 25190-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RSL24D1 antibody (25190-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

RSL24D1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25190-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C15orf15, HRP L30 iso, L30, My024, Ribosomal L24 domain-containing protein 1

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Tested Applications

• Positive WB detected in: HepG2 cells, HL-60 cells, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 3 publications below

Product Information

25190-1-AP targets RSL24D1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSL24D1 fusion protein Ag18195
• Full Name: ribosomal L24 domain containing 1
• Calculated Molecular Weight: 163 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC008409
• Gene Symbol: RSL24D1
• Gene ID (NCBI): 51187
• RRID: AB_2879950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHA3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSL24D1 (Ribosomal L24 Domain Containing 1) is a ribosomal protein that is primarily localized in the nucleolus and is involved in the biogenesis of the 60S ribosomal subunit. In mouse embryonic stem cells (ESCs), the expression level of RSL24D1 is high and significantly decreases during cell differentiation. The role of RSL24D1 in ribosome biogenesis includes facilitating proper protein docking in nucleolar and cytoplasmic precursor particles. After the completion of biogenesis, RSL24D1 dissociates from the particles and is thought to be replaced by ribosomal protein L24.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Cell Rep; Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly.; Authors - Chunyang Ni
human	WB	RNA; Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing; Authors - Mason A McCool
human	IF	mBio; Genome-Scale CRISPR Screening Reveals Host Factors Required for Ribosome Formation and Viral Replication; Authors - Maikke B Ohlson
human	WB	Cell; The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity; Authors - Vidhya Krishnamoorthy
human	WB,IF	Nat Cell Biol; A programmed decline in ribosome levels governs human early neurodevelopment; Authors - Chunyang Ni