RSPH1 Polyclonal antibody

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  WB analysis using 32033-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32033-1-AP (RSPH1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

RSPH1 Polyclonal Antibody for WB, ELISA

Cat No. 32033-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Radial Spoke Head Component 1, Radial spoke head 1 homolog, Meichroacidin, Male meiotic metaphase chromosome-associated acidic protein, CT79

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Tested Applications

• Positive WB detected in: human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32033-1-AP targets RSPH1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSPH1 fusion protein Ag36560
• Full Name: radial spoke head 1 homolog (Chlamydomonas)
• Calculated Molecular Weight: 309 aa, 35 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC101519
• Gene Symbol: RSPH1
• Gene ID (NCBI): 89765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8WYR4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSPH1 (Radial Spoke Head Component 1) encodes a male mid-meiotic chromosome-associated acidic protein involved in the structure and function of cilia and flagella. Pathogenic variants of RSPH1 lead to impaired spermatocyte motility and aberrant flagellar composition, which further contribute to male infertility. In addition to this, mutations in the RSPH1 gene lead to primary ciliary dyskinesia-24 (PCD-24), an autosomal recessive disorder that affects the motor function of cilia and flagella.