Recombinant Rat CD4 protein (rFc Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2025

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Validation Data Gallery

• 

  Purity of Recombinant Rat CD4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat CD4 protein Lys28-Thr394 (Accession# P05540) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 24932
• Accession: P05540
• PredictedSize: 67.4 kDa
• SDS-PAGE: 70-85 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV). CD4 triggering by gp120, a natural ligand of CD4, potentiates CD44-mediated adhesion to hyaluronic acid. CD4 is present in human and rat macrophages, but absent in mice.

References:

1. K Bowers, et al. (1997) Int J Biochem Cell Biol. 29(6):871-5.
2. Gina Graziani-Bowering, et al. (2002) Exp Cell Res. 279(1):141-52.
3. J M Riberdy, et al. (1998) Proc Natl Acad Sci U S A. 95(8):4493-8.
4. U Dianzani, et al. (1999) Int Immunol. 11(7):1085-92.
5. P R Crocker, et al. (1987) J Exp Med. 166(2):613-8.