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Recombinant Rat CCL2/MCP-1 protein (His Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

C-His

Activity

not tested

Cat no : Eg0397

Validation Data Gallery

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat CCL2 protein Gln24-Asn148 (Accession# P14844) with a His tag at the C-terminus.
GeneID 24770
Accession P14844
PredictedSize 17.9 kDa
SDS-PAGE 40-50 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

References:

1. Sørensen T. et al. (2004) Eur J Neurol. 11: 445-9.
2. Kusano KF. et al. (2004) Circ J. 68: 671-6.
3. Hayashida K. et al. (2001) Arthritis Res. 3: 118-26.
4. Dimitrijevic OB. et al. (2006) J Cereb Blood Flow Metab. 26:797-8105. Mahad DJ. et al. (2003) Semin Immunol. 15:23-32.