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Recombinant Rat RANK/TNFRSF11A protein (rFc Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3285

Validation Data Gallery

  • Purity of Recombinant Rat RANK was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat RANK was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat RANK protein Val31-Gln209 (Accession# F1M8Z6) with a rabbit IgG Fc tag at the C-terminus.
GeneID 498206
Accession F1M8Z6
PredictedSize 45.9 kDa
SDS-PAGE 48-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TNFRSF11A (tumor necrosis factor receptor superfamily, member 11A), also known as receptor activator of NF-κB (RANK), is highly expressed in bone and has an important role in osteoclast differentiation and activity. Tnfrsf11a deficiency in mice has also been associated with severe abnormalities in thymic epithelial cells and impaired negative selection, with severe autoimmune manifestations. Overexpression of RANK/TNFRSF11A in glioblastoma cell lines leads to a significant reduction in focus formation and elevated apoptotic activity.

References:

1. Hughes A.E., et al. (2000). Nat Genet. Jan;24(1):45-48. 
2. von dem Knesebeck A, et al. (2012). Neoplasia. Jun;14(6):526-34.
3. Guerrini M.M., et al. (2008). Am J Hum Genet. Jul;83(1):64-76.