S100A4 Polyclonal antibody

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  WB analysis using 16105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16105-1-AP (S100A4 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16105-1-AP

  WB result of S100A4 antibody (16105-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A4 transfected HeLa cells.

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  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse spleen using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16105-1-AP (S100A4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 16105-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100A4 antibody (16105-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 16105-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FSP1/S100A4 Polyclonal antibody (16105-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

S100A4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 16105-1-AP

Publications(65)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

FSP1, Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein, CAPL

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, human pancreas cancer tissue, human colon cancer tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 30 publications below
• IHC: See 18 publications below
• IF: See 32 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Product Information

16105-1-AP targets S100A4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A4 fusion protein Ag9019
• Full Name: S100 calcium binding protein A4
• Calculated Molecular Weight: 101 aa, 12 kDa
• Observed Molecular Weight: 10-12 kDa
• GenBank Accession Number: BC016300
• Gene Symbol: S100A4
• Gene ID (NCBI): 6275
• ENSEMBL Gene ID: ENSG00000196154
• RRID: AB_11042591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P26447
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Publications

Species	Application	Title
human	WB	Cell; Suppression of Enhancer Overactivation by a RACK7-Histone Demethylase Complex.; Authors - Hongjie Shen ; KO Validated
human	IHC	J Hematol Oncol; Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma; Authors - Yexin Tong
human	WB	Nat Commun; Co-evolution of matrisome and adaptive adhesion dynamics drives ovarian cancer chemoresistance.; Authors - Elina A Pietilä
mouse	WB	ACS Appl Mater Interfaces; Smart Manganese Dioxide-Based Lanthanide Nanoprobes for Triple-Negative Breast Cancer Precise Gene Synergistic Chemodynamic Therapy.; Authors - Liyan Ming
human	WB,IF	Oncogene; Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids; Authors - Hui Zhao
human	IF	J Crohns Colitis; Exosomal miR-103a-3p from Crohn's creeping fat-derived ASCs contributes to intestinal fibrosis by targeting TGFBR3 and activating fibroblasts; Authors - Wenwei Qian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-09-2024)

Does exactly what it should do in cardiac rat cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs