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S100A4 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 16105-1-AP

Featured Product KD/KO validated

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Con: 34 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

S100A4 Antibody WB HeLa cells 16105-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 16105-1-AP(S100A4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

S100A4 Antibody WB HeLa cells 16105-1-AP

WB result of S100A4 antibody (16105-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A4 transfected HeLa cells

S100A4 Antibody IHC human breast cancer tissue 16105-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16105-1-AP( S100A4 Antibody) at dilution of 1:200 (under 10x lens).

S100A4 Antibody IHC human breast cancer tissue 16105-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16105-1-AP( S100A4 Antibody) at dilution of 1:200 (under 40x lens).

S100A4 Antibody IF HeLa cells 16105-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16105-1-AP(S100A4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
HeLa cells, NIH/3T3 cells
Positive IHC detected in:
human breast cancer tissue, human colon cancer tissue, human pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
S100A4 fusion protein Ag9019
Full name:
S100 calcium binding protein A4
Calculated molecular weight:
101aa,12 kDa
Observed molecular weight:
10-12 kDa
GenBank accession number:
BC016300
Gene ID (NCBI):
6275
Gene symbol
S100A4
Synonyms
18A2, 42A, Calvasculin, CAPL, Fibroblast Specific Protein, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4

Background

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.


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