S100A4 Polyclonal antibody

• Featured Product
• KD/KO Validated

S100A4 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

30

Cat no : 16105-1-AP

Synonyms

18A2, 42A, Calvasculin, CAPL, Fibroblast Specific Protein, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4

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Validation Data Gallery

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  WB analysis using 16105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16105-1-AP (S100A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16105-1-AP

  WB result of S100A4 antibody (16105-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A4 transfected HeLa cells.

• 

  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16105-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16105-1-AP (S100A4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 16105-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human S100A4 (16105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 11 publications below
• IF: See 12 publications below
• IP: See 1 publications below

Product Information

The immunogen of 16105-1-AP is S100A4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A4 fusion protein Ag9019
• Full Name: S100 calcium binding protein A4
• Calculated molecular weight: 101 aa, 12 kDa
• Observed molecular weight: 10-12 kDa
• GenBank accession number: BC016300
• Gene symbol: S100A4
• Gene ID (NCBI): 6275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Publications

Species	Application	Title
mouse	IHC	Nat Commun; Kindlin-2 links mechano-environment to proline synthesis and tumor growth.; Authors - Ling Guo
human	WB,IHC,IF	Cell Death Dis; PMID: 31024010; Authors - Dong-Ming Wu
human	WB	Br J Cancer; ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins.; Authors - Qais Al-Ismaeel ; KD Validated
mouse	WB	ACS Appl Mater Interfaces; Smart Manganese Dioxide-Based Lanthanide Nanoprobes for Triple-Negative Breast Cancer Precise Gene Synergistic Chemodynamic Therapy.; Authors - Liyan Ming
human	WB,IF	ACS Biomater Sci Eng; LINC01915 Facilitates the Conversion of Normal Fibroblasts into Cancer-Associated Fibroblasts Induced by Colorectal Cancer-Derived Extracellular Vesicles through the miR-92a-3p/KLF4/CH25H Axis.; Authors - Minghe Zhou
human	IHC	Anat Rec (Hoboken); Inversed Expression Patterns of S100A4 and E-Cadherin in Cervical Cancers: Implication in Epithelial-Mesenchymal Transition.; Authors - Ming Liu

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