S100A4 Monoclonal antibody

• Featured Product
• KD/KO Validated

S100A4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

2G11B4

Cat no : 66489-1-Ig

Synonyms

18A2, 42A, Calvasculin, CAPL, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4

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Validation Data Gallery

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  WB analysis of HeLa using 66489-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66489-1-Ig

  WB result of S100A4 antibody (66489-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A4 transfected HeLa cells.

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  WB analysis of A549 using 66489-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 66489-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66489-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66489-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of K-562 using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded k562 slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66489-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66489-1-Ig (S100A4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, A375 cells, PC-3 cells, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, human tonsillitis tissue, K-562 cells, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Product Information

66489-1-Ig targets S100A4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: S100A4 fusion protein Ag9019
• Full Name: S100 calcium binding protein A4
• Calculated molecular weight: 101 aa, 12 kDa
• Observed molecular weight: 12 kDa
• GenBank accession number: BC016300
• Gene symbol: S100A4
• Gene ID (NCBI): 6275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Publications

Species	Application	Title
human	WB	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
mouse	IHC	Cell Death Differ; Upregulation of Wilms' Tumor 1 in epicardial cells increases cardiac fibrosis in dystrophic mice.; Authors - Zhenglong Guo
mouse	IF,FC	PLoS Pathog; S100A4+ macrophages facilitate zika virus invasion and persistence in the seminiferous tubules via interferon-gamma mediation.; Authors - Wei Yang
mouse	WB	Cancers (Basel); Usp22 Overexpression Leads to Aberrant Signal Transduction of Cancer-Related Pathways but Is Not Sufficient to Drive Tumor Formation in Mice; Authors - Xianghong Kuang
rat	WB,IF	J Cell Mol Med; Polydatin inhibits ZEB1-invoked epithelial-mesenchymal transition in fructose-induced liver fibrosis.; Authors - Xiaojuan Zhao
human	WB,IHC,IF	Cell Commun Signal; A functional role of S100A4/non-muscle myosin IIA axis for pro-tumorigenic vascular functions in glioblastoma.; Authors - Madoca Inukai ; KD Validated