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S100A4 Monoclonal antibody
S100A4 Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66489-1-Ig
Validation Data Gallery
|Positive WB detected in
|HeLa cells, A549 cells, A375 cells, PC-3 cells, NIH/3T3 cells
|Positive IHC detected in
|human lung cancer tissue, human colon cancer tissue, human tonsillitis tissue, K-562 cells, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HepG2 cells, HeLa cells
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:1000-1:4000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66489-1-Ig targets S100A4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Mouse / IgG2a
|S100A4 fusion protein Ag9019
|S100 calcium binding protein A4
|Calculated molecular weight
|101 aa, 12 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.
C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse
Cell Death Differ
Upregulation of Wilms' Tumor 1 in epicardial cells increases cardiac fibrosis in dystrophic mice.
S100A4+ macrophages facilitate zika virus invasion and persistence in the seminiferous tubules via interferon-gamma mediation.
Usp22 Overexpression Leads to Aberrant Signal Transduction of Cancer-Related Pathways but Is Not Sufficient to Drive Tumor Formation in Mice
Altered placental ion channel gene expression in preeclamptic high-altitude pregnancies
J Cell Mol Med
Polydatin inhibits ZEB1-invoked epithelial-mesenchymal transition in fructose-induced liver fibrosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Shinford (Verified Customer) (10-26-2023)
I have some reservations about the antibody because of its relatively low molecular weight. We attempted to create separate gels with 10% and 12% concentrations and made an effort to run the gels for a limited time to ensure that the entire protein remained within the original gels and membranes. While we were able to detect several bands, their positions lower in the gel led to less than ideal band shapes.