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S100A4 Monoclonal antibody

S100A4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2G11B4

Cat no : 66489-1-Ig

Synonyms

18A2, 42A, Calvasculin, CAPL, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4



Tested Applications

Positive WB detected inHeLa cells, A549 cells, A375 cells, PC-3 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human tonsillitis tissue, K-562 cells, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66489-1-Ig targets S100A4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen S100A4 fusion protein Ag9019
Full Name S100 calcium binding protein A4
Calculated molecular weight 101 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC016300
Gene symbol S100A4
Gene ID (NCBI) 6275
RRIDAB_2881854
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Protocols

Product Specific Protocols
WB protocol for S100A4 antibody 66489-1-IgDownload protocol
IHC protocol for S100A4 antibody 66489-1-IgDownload protocol
IF protocol for S100A4 antibody 66489-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Blood

C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse

Authors - Li-Xue Yang
mouseIHC

Cell Death Differ

Upregulation of Wilms' Tumor 1 in epicardial cells increases cardiac fibrosis in dystrophic mice.

Authors - Zhenglong Guo
mouseIF,FC

PLoS Pathog

S100A4+ macrophages facilitate zika virus invasion and persistence in the seminiferous tubules via interferon-gamma mediation.

Authors - Wei Yang
mouseWB

Cancers (Basel)

Usp22 Overexpression Leads to Aberrant Signal Transduction of Cancer-Related Pathways but Is Not Sufficient to Drive Tumor Formation in Mice

Authors - Xianghong Kuang
humanIF

Physiol Genomics

Altered placental ion channel gene expression in preeclamptic high-altitude pregnancies

Authors - Colleen G Julian
ratWB,IF

J Cell Mol Med

Polydatin inhibits ZEB1-invoked epithelial-mesenchymal transition in fructose-induced liver fibrosis.

Authors - Xiaojuan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (10-26-2023)

I have some reservations about the antibody because of its relatively low molecular weight. We attempted to create separate gels with 10% and 12% concentrations and made an effort to run the gels for a limited time to ensure that the entire protein remained within the original gels and membranes. While we were able to detect several bands, their positions lower in the gel led to less than ideal band shapes.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Lung cancer and breast cancer cell lines
S100A4 Antibody Western Blot validation (1:500 dilution) in Lung cancer and breast cancer cell lines (Cat no:66489-1-Ig)