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SAMHD1 Polyclonal antibody
SAMHD1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 12586-1-AP
|Positive WB detected in||HepG2 cells, human brain tissue, human heart tissue, K-562 cells, L02 cells, mouse heart tissue|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human lung cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12586-1-AP targets SAMHD1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SAMHD1 fusion protein Ag3287|
|Full Name||SAM domain and HD domain 1|
|Calculated molecular weight||626 aa, 72 kDa|
|Observed molecular weight||64-72 kDa|
|GenBank accession number||BC036450|
|Gene ID (NCBI)||25939|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with a presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.
|Product Specific Protocols|
|WB protocol for SAMHD1 antibody 12586-1-AP||Download protocol|
|IHC protocol for SAMHD1 antibody 12586-1-AP||Download protocol|
|IF protocol for SAMHD1 antibody 12586-1-AP||Download protocol|
|IP protocol for SAMHD1 antibody 12586-1-AP||Download protocol|
|FC protocol for SAMHD1 antibody 12586-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochem Biophys Res Commun
DNA damage response signaling does not trigger redistribution of SAMHD1 to nuclear foci.
Antimicrob Agents Chemother
SAMHD1 specifically affects the antiviral potency of thymidine analog HIV reverse transcriptase inhibitors.
Palbociclib, a selective inhibitor of cyclin-dependent kinase4/6, blocks HIV-1 reverse transcription through the control of sterile α motif and HD domain-containing protein-1 activity.
Mol Med Rep
Interferon-stimulated gene factor 3 complex is required for the induction of sterile α motif and HD domain-containing protein 1 expression by interferon-α in SMMC-7721 cells.
Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97.