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Secretogranin III Polyclonal antibody

Secretogranin III Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10954-1-AP

Synonyms

SCG3, Secretogranin 3, secretogranin III, SGIII



Tested Applications

Positive IHC detected inmouse pancreas tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10954-1-AP targets Secretogranin III in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Secretogranin III fusion protein Ag1397
Full Name secretogranin III
Calculated molecular weight 53 kDa
GenBank accession numberBC014539
Gene symbol SCG3
Gene ID (NCBI) 29106
RRIDAB_2190449
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Secretogranin III (SCG3), a member of the granin protein family, is expressed specifically in neuronal and endocrine cells. SCG3 was synthesized as a 61- or 63-kDa protein and processed to a 48-kDa form which, in turn, was partially cleaved to fragments of 28 and 20 kDa.

Protocols

Product Specific Protocols
IHC protocol for Secretogranin III antibody 10954-1-APDownload protocol
IF protocol for Secretogranin III antibody 10954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Exp Med

Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.

Authors - Michelle E LeBlanc
humanWB,IHC

Cancer Lett

The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells.

Authors - Yue Wang
  • KD Validated
mouseWB,IHC

Cereb Cortex

Secretogranin III is an astrocyte granin that is overexpressed in reactive glia.

Authors - Paco Sonia S
human, mouseIHC

Brain Pathol

Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.

Authors - Virginia Plá
mouseIHC,IF

J Endocrinol

Secretogranin II binds to secretogranin III and forms secretory granules with orexin, neuropeptide Y, and POMC.

Authors - Hotta Kikuko K
IF,WB

Adv Sci (Weinh)

Tuning the Size of Large Dense-Core Vesicles and Quantal Neurotransmitter Release via Secretogranin II Liquid-Liquid Phase Separation.

Authors - Zhaohan Lin
  • KD Validated