SCN4A Recombinant antibody

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  WB analysis using 85698-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85698-1-RR (SCN4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 85698-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85698-1-RR (SCN4A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 85698-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85698-1-RR (SCN4A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SCN4A Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85698-1-RR

Clone No.243036A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

HYKPP, HYPP, Na(V)1.4, NAC1A, Nav1.4

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells, COLO 205 cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85698-1-RR targets SCN4A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: sodium channel, voltage-gated, type IV, alpha subunit
• Calculated Molecular Weight: 208 kDa
• Observed Molecular Weight: 208 kDa
• GenBank Accession Number: NM_000334
• Gene Symbol: SCN4A
• Gene ID (NCBI): 6329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35499
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sodium channel protein type 4 subunit alpha (SCN4A, also known as Nav1.4) is the pore-forming subunit of the primary sodium channel present in skeletal muscles (PMID: 16193245). Nav1.4 related channelopathies that affect skeletal muscle excitability are dominant diseases classified in two opposite groups as defined by the prevalent clinical symptoms: muscle stiffness and hypertonia (myotonia) episodes [non dystrophic myotonias (NDM)], and muscle weakness resulting in paralysis episodes (periodic paralyses; PP) (PMID: 20237798; 26285000).