SCN4A Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85698-1-RR (same clone as 85698-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85698-1-RR (SCN4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85698-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 85698-1-RR (same clone as 85698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85698-1-RR (SCN4A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85698-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 85698-1-RR (same clone as 85698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85698-1-RR (SCN4A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85698-1-PBS in a different storage buffer formulation.

SCN4A Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85698-1-PBS

Clone No.243036A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

HYKPP, HYPP, Na(V)1.4, NAC1A, Nav1.4

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Product Information

85698-1-PBS targets SCN4A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: sodium channel, voltage-gated, type IV, alpha subunit
• Calculated Molecular Weight: 208 kDa
• Observed Molecular Weight: 208 kDa
• GenBank Accession Number: NM_000334
• Gene Symbol: SCN4A
• Gene ID (NCBI): 6329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35499
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sodium channel protein type 4 subunit alpha (SCN4A, also known as Nav1.4) is the pore-forming subunit of the primary sodium channel present in skeletal muscles (PMID: 16193245). Nav1.4 related channelopathies that affect skeletal muscle excitability are dominant diseases classified in two opposite groups as defined by the prevalent clinical symptoms: muscle stiffness and hypertonia (myotonia) episodes [non dystrophic myotonias (NDM)], and muscle weakness resulting in paralysis episodes (periodic paralyses; PP) (PMID: 20237798; 26285000).